LOCUS       sequence-135655-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human PBX2 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_PBX2_2
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..128
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             135..155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttaattacc tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca
       61 tggactataa ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg
      121 acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
      181 acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca
      241 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
      301 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
      361 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
      421 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
      481 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
      541 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
      601 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
      661 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
      721 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
      781 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc
      841 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga
      901 ttgccctgag cctgggcctg acccc
//
