LOCUS       sequence-135662-         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_TGIF2_2
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        112..360
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     112..132
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     283..302
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        379..454
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tgagggccta
      121 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
      181 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
      241 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
      301 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
      361 gtaggcatct gccaagaagt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
      421 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gttttagagc tagaaatagc
      481 aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca attctgcaga caaatggctc
      541 tagaggtacc cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      601 cccgcccatt gacgtcaata gtaacgccaa tagggacttt ccattgac
//
