LOCUS       sequence-135665-        9591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  localization of yeast RNase RMP1 (RNase MRP binding protein) in 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Gill T, Aulds J, Schmitt ME
  TITLE     A specialized processing body that is temporally and asymmetrically 
            regulated during the cell cycle in Saccharomyces cerevisiae.
  JOURNAL   J Cell Biol. 2006 Apr 10;173(1):35-45. Epub 2006 Apr 3.
  PUBMED    16585272
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2006-04-10"; volume: "173(1)"; pages: "35-45. Epub 
            2006 Apr 3"
COMMENT     Sequence Label: pTG005
FEATURES             Location/Qualifiers
     source          1..9591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             481..1194
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(511..539)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1134..1154
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        3097..3317
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3318..4121
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    5466..5758
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      6852..7305
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(7333..7355)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7441..7581
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7496..7515
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7596..7613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7767..8355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7847..7866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8526..9317,9318..9386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9149..9168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9387..9491)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9559..9577
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattctcaa aacccttaaa aacatatgcc tcaccctaac atattttcca attaaccctc
       61 aatatttctc tgtcacccgg cctctatttt ccattttctt ctttacccgc cacgcgtttt
      121 tttctttcaa atttttttct tccttcttct ttttcttcca cgtcctcttg cataaataaa
      181 taaaccgttt tgaaaccaaa ctcgcctctc tctctccttt ttgaaatatt tttgggtttg
      241 tttgatcctt tccttcccaa tctctcttgt ttaatatata ttcatttata tcacgctctc
      301 tttttatctt cctttttttc ctctctcttg tattcttcct tcccctttct actcaaacca
      361 agaagaaaaa gaaaaggtca atctttgtta aagaatagga tcttctacta catcagcttt
      421 tagatttttc acgcttactg cttttttctt cccaagatcg aaaatttacg gatctcaaag
      481 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      541 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      601 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      661 gtcactactt tcacttatgg tgttcaatgc ttttcaagat acccagatca tatgaaacgg
      721 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      781 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      841 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      901 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      961 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     1021 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1081 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     1141 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaaggatcc
     1201 atggatgaga tggataatgt gatacgatct ctggagcaag aatatcggtt aatattgctc
     1261 ttgaaccata ggaacaaaaa tcaacataga gcggctagct ggtatggatc gttcaatgag
     1321 atgaaaagaa attgtggaca aataataacg ctttttagct cgagaagatt acaagccaaa
     1381 cgccttaaag atgttgaatg ggtcaagttg cacaggctat tacagagagc actttttaga
     1441 cagttaaaga gatggtactg gcagttcaat ggcgtaattg cgctgggaca atttgtaacg
     1501 ttgggttgta cactagtaac attgctggca aatgtgaggg cactgtatat gagattatgg
     1561 gagattaatg aaactgagtt tataagatgt ggatgcttaa taaagaactt accgagaaca
     1621 aaagcaaaat cggttgtgaa cgatgtcgaa gaacttggag aaattataga tgaagatatt
     1681 ggcaacaacg ttcaagaaaa cgaactagtg ataacgtcta taccaaagcc tctgacagag
     1741 aactgtaaga agaaaaagaa aaggaaaaag aagaacaaat cagccattga tggcatattc
     1801 ggataagctt gctctagata atctctgctt ttgtgcgcgc atgtttatgt atgtacctct
     1861 ctctctattt ctatttttaa accaccctct caataaaata aaaataataa agtattttta
     1921 aggaaaagac gtgtttaagc actgacttta tctacttttt gtacgttttc attgatataa
     1981 tgtgttttgt ctctcccttt tctacgaaaa tttcaaaaat tgaccaaaaa aaggaatata
     2041 tatacgaaaa actattatat ttatatatca tagtgttgat aaaaaatgtt tatccattgg
     2101 accgtgtatc atatgatcat ctcttcatcg cttttcagtt ttataggttt atgcaattgc
     2161 ccttcttggg aggatcttgt agaaccgcca ttagaatttg agttcggcgt tttgataccc
     2221 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     2281 tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc
     2341 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     2401 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     2461 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     2521 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     2581 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     2641 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     2701 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     2761 cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt
     2821 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
     2881 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
     2941 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
     3001 gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat
     3061 ctccagcagc cgcacgcggc gcatcggggg gggggtttca attcaattca tcattttttt
     3121 tttattcttt tttttgattt cggtttcttt gaaatttttt tgattcggta atctccgaac
     3181 agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg
     3241 ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca
     3301 ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta
     3361 gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt
     3421 cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa
     3481 tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta
     3541 agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg
     3601 ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat
     3661 gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc
     3721 aggcggcaga agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat
     3781 gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg
     3841 acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt
     3901 acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc
     3961 aacagtatag aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa
     4021 gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag
     4081 gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa
     4141 atgcatgtat actaaactca caaattagag cttcaattta attatatcag ttattacccg
     4201 ggaatctcgg tcgtaatgat ttttataatg acgaaaaaaa aaaaattgga aagaaaaccc
     4261 cccccccgca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg
     4321 acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag
     4381 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc
     4441 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg
     4501 ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta
     4561 acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc
     4621 agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc
     4681 gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa ttccccctta
     4741 cacggaggca tcaagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
     4801 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
     4861 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcaggtgg gccattctca
     4921 tgaagaatat cttgaattta ttgtcatatt actagttggt gtggaagtcc atatatcggt
     4981 gatcaatata gtggttgaca tgctggctag tcaacattga gccttttgat catgcaaata
     5041 tattacggta ttttacaatc aaatatcaaa cttaactatt gactttataa cttatttagg
     5101 tggtaacatt cttataaaaa agaaaaaaat tactgcaaaa cagtactagc ttttaacttg
     5161 tatcctaggt tatctatgct gtctcaccat agagaatatt acctatttca gaatgtatgt
     5221 ccatgattcg ccgggtaaat acatataata cacaaatctg gcttaataaa gtctataata
     5281 tatctcataa agaagtgcta aattggctag tgctatatat ttttaagaaa atttcttttg
     5341 actaagtcca tatcgacttt gtaaaagttc actttagcat acatatatta cacgagccag
     5401 aaattgtaac ttttgcctaa aatcacaaat tgcaaaattt aattgcttgc aaaaggtcac
     5461 atgcttataa tcaacttttt taaaaattta aaatactttt ttatttttta tttttaaaca
     5521 taaatgaaat aatttattta ttgtttatga ttaccgaaac ataaaacctg ctcaagaaaa
     5581 agaaactgtt ttgtccttgg aaaaaaagca ctacctagga gcggccaaaa tgccgaggct
     5641 ttcatagctt aaactcttta cagaaaatag gcattataga tcagttcgag ttttcttatt
     5701 cttccttccg gttttatcgt cacagtttta cagtaaataa gtatcacctc ttagagttaa
     5761 ctatgagata agcaagtatc atctcatttc atttacctga agtcgagtaa acagaaaatc
     5821 caattgttga tgaacctcaa tgacttagaa ctatctatcg gcagatcata taaagaggat
     5881 ttaggtacct agaggactgt acctggagta tatatatata tatatatata ttatctcaac
     5941 tatagtccat agaggtttct ttcttgaggc cttaaactgc taaagaatga tattggtgga
     6001 atgcaagcac caatctctct tctttcgtaa ctgttcatat acttcaaacc aagaatgtaa
     6061 cgggcattga cccatccaaa accttcagta gctgcccctt taaagtcagc accttgatta
     6121 ccgtattctg cttcaacacg atgaggatct gttcctcttg tgacatcata tttttcaacc
     6181 acaataccat tataatcgac aaaagccttt gtcatcatga aaagccatct ataagctagc
     6241 ctattcgtta cagttaaata accataagaa cggaggcctt cccaagcaag aatttgatgg
     6301 ggtgcccaac caaatggata gtcccattgt ctaattggtc tcgaaataga aattgggcct
     6361 cgagaacgct ccgtacatgc agctaaacct ccaagcatct ctaacttggg tagtgctttc
     6421 tccaccattt tctgtgcttg ctccttcgtg gcaagtccag cccataatgc ccagaatgta
     6481 gttgcggatt cgtatgacgt tctgtgcttg atttttgtgt tgtagtcaaa gaaaaggtcc
     6541 ggttaaacgg atctcgcatt gatgaggcaa cgctaattat caacatatag attgttatct
     6601 atctgcatga acacgaaatc tttacttgac gacttgaggc tgatggtgtt tatgcaaaga
     6661 aaccactgtg tttaatatgt gtcactgttt gatattactg tcagcgtaga agataatagt
     6721 aaaagcggtt aataagtgta tttgagataa gtgtgataaa gtttttacag cgaaaagacg
     6781 ataaatacaa gaaaatgatt acgaggatac ggagagaggt atgtacatgt gtatttatat
     6841 actaagctgc cggcggttgt ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg
     6901 tagcgtatgc gcctgtgaac attctcttca acaagtttga ttccattgcg gtgaaatggt
     6961 aaaagtcaac cccctgcgat gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt
     7021 tagcattatc tttacatctt gttattttac agattttatg tttagatctt ttatgcttgc
     7081 ttttcaaaag gcctgcaggc aagtgcacaa acaatactta aataaatact actcagtaat
     7141 aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt
     7201 tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc
     7261 aacattttct ggcgtcagtc caccagctaa cataaaatgt tgcctcgcgc gtttcggtga
     7321 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     7381 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     7441 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     7501 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     7561 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7621 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7681 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7741 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7801 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7861 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7921 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7981 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8041 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8101 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8161 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8221 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8281 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8341 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8401 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8461 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8521 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8581 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8641 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8701 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8761 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8821 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8881 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8941 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9001 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9061 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9121 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     9181 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9241 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9301 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9361 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9421 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9481 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9541 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca a
//
