LOCUS       sequence-135667-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-YNL
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..43
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     polyA_signal    90..314
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     303..320
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca tgctccagac tgccttggga
       61 aaagctgcag gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct
      121 gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
      181 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
      241 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
      301 actagaaggc acagtcgagg ctgatcagcg agctctagat catcgatcaa gcttatcgag
      361 cggccgctct agattactgc tcgttcttca gcactctctc cacgaagctc ttgatgtact
      421 tgcccatctc gtcgggggcg tcctcctgga ggaagtgcag gcccttcacc ttcacgaact
      481 cggtgttggg gaacttcttg gcgccctcca cgatggcgtt gctgaagaag ccggggtcgc
      541 cctcgatgaa cagcttgggc aggtcgtcgc tggctctcag gtaggcgttg tagtttctca
      601 cgatctgcac cacgtcgggc ttgccgccct tcaccagggg gatctctctg ggccagctca
      661 gggtgggtct tctcacctcg cccttctcct tgaagggctc caggtaggcg gcgaactcct
      721 cgggctccag ctttctcatg atcttgctgg gcagcacggt ctccacgaag aagttgttct
      781 ccagcaccat cttctcgccc tcctcgctct tgatcagggc gatgtcctcc tcgatgtctg
      841 gccactcgtc ccagctctcg atcacgtcca ccacgctctc catgtgcacg atggccttga
      901 tcctgtcctg gtgctcgtag gcgtagtgga aggccagggc ggcgccccag tcgtggccca
      961 cgaagatgat cttcttgggc aggttcagaa g
//
