LOCUS       sequence-135682-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, His40>Ala/Phe79>Trp mutant (TdPurE2-H40A,F79W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Kappock
  TITLE     Serine mutants of Acetobacter aceti PurE1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK657
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..23)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             584..606
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac ggagctcgaa
       61 ttcgagctcc gtcgacaaga aactgaaacg ctgaattcag cgtttcagtt tcgaatcgtc
      121 ttcgataatt ttctgcgcat tggattccat ataggatttg accgaatctg cgatttcttt
      181 atcgtacagt gaaaaaatac gagccgccag gagtgccgca tttttcggtt caagaaccag
      241 tgccggacta atcccgctcg gcatccgtaa cgaagaataa atgtcggcgc cggcgaagct
      301 atcgcttggc ggtgggcagg caatggttgc acctttgacc cagccatcaa caaagcctga
      361 taaagcgtta ctacgcccag cgatcgtgat gtacagttta ggacggtcca aagcttcata
      421 ttctttcagc atagacacta catgctccgc tgttttagcc gcggagccga tgcgaatggc
      481 atactcaata ccaaaagttt tcaattcaga cgcaattttc tcggcgtgac ccatatctga
      541 actgctgccc ataaggataa tcaccagcgg acgcatatgt atatctcctt cttaaagtta
      601 aacaaaatta tttctagagg gaaaccgttg tggtctccct ata
//
