LOCUS       sequence-135685-         541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, His40>Asn/Phe79>Trp mutant (TdPurE2-H40N,F79W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Kappock
  TITLE     Serine mutants of Acetobacter aceti PurE1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK658
FEATURES             Location/Qualifiers
     source          1..541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgcgtc cgctggtgat tatccttatg
       61 ggcagcagtt cagatatggg tcacgccgag aaaattgcgt ctgaattgaa aacttttggt
      121 attgagtatg ccattcgcat cggctccgcg aataaaacag cggagcatgt agtgtctatg
      181 ctgaaagaat atgaagcttt ggaccgtcct aaactgtaca tcacgatcgc tgggcgtagt
      241 aacgctttat caggctttgt tgatggctgg gtcaaaggtg caaccattgc ctgcccaccg
      301 ccaagcgata gcttcgccgg cgccgacatt tattcttcgt tacggatgcc gagcgggatt
      361 agtccggcac tggttcttga accgaaaaat gcggcactcc tggcggctcg tattttttca
      421 ctgtacgata aagaaatcgc agattcggtc aaatcctata tggaatccaa tgcgcagaaa
      481 attatcgaag acgattcgaa actgaaacgc tgaattcagc gtttcagttt cttgtcgacg
      541 g
//
