LOCUS       sequence-135693-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.
            A retroviral expression vector with the neomycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H
  TITLE     Identification of telomere-associated molecules by engineered 
            DNA-binding molecule-mediated chromatin immunoprecipitation 
            (enChIP).
  JOURNAL   Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038/srep03171.
  PUBMED    24201379
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-8"; volume: "3:3171. doi"; pages: " 
            10.1038/srep03171"
COMMENT     Sequence Label: 3xFN-Tel-TAL/pMXs-neo
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             117..182
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      198..207
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             207..248
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             261..281
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aggctgccga ccccgggggt ggaccatcct ctagactgcc ggatctagct agttaattaa
       61 ggatcccagt gtggtggtac gggaattcgt ttagtgaacc gtcagaatta accatggact
      121 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
      181 agcttgcggc cggccgcgcc accatgggaa aacctattcc taatcctctg ctgggcctgg
      241 attctaccgg aggcatggcc cctaagaaaa agcggaaggt ggacggcgga gtggacctga
      301 gaacactggg atattctcag cagcagcagg agaagatcaa gcccaaggtg agatccacag
      361 tggcccagca ccacgaagcc ctggtgggac acggatttac acacgcccac attgtggccc
      421 tgtctcagca ccctgccgcc ctgggaacag tggccgtgaa atatcaggat atgattgccg
      481 ccctgcctga ggccacacac gaagccattg tgggagtggg aaaacagtgg tctggagcca
      541 gagccctgga agccctgctg acagtggccg gagaactgag aggacctcct ctgcagctgg
      601 atacaggaca gctgctgaag attgccaaaa ggggcggagt gaccgcggtg gaagccgtgc
      661 acgcctggag aaatgccctg acaggagccc ctctgaacct gacccccgaa caggtggtgg
      721 ccattgccag caacatcggc ggcaagcagg ccctggaaac cgtgcagaga ctgctgcccg
      781 tgctgtgcca ggcccatggc ctgacacctg aacaggtggt ggctatcgcc tctaacaacg
      841 gaggaaaaca ggctctggaa acagtgcagc ggctgctgcc tgtgctgtgt caggctcacg
      901 gcttgactcc agaacaggtg gtggctattg cttccaacaa cggggggaaa caggccctgg
      961 aaactgtgca gcgcctgctg ccagtgctgt gccaggctca cggactgacc cccgaacagg
     1021 tggtggccat tgccagcaac aacggcggca agcag
//
