LOCUS       sequence-135703-       16116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry and FlpO for monosynaptic circuit mapping and 
            genetic manipulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16116)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 16116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO
FEATURES             Location/Qualifiers
     source          1..16116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3325..4032
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3470..3488)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3738..3757
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4042..4098
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7545..7556
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9348..9359)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12556..12574)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12570..12617
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12645..12661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12645..12661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12658..12680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12669..12685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12693..12699,12700..12717,12718..12723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12738..12759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12876..12893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13047..13635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13127..13146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13806..14597,14598..14666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14429..14448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14667..14771)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14798..15253
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14885..14904)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15095..15116
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15379..15401
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15393..15410
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15394..15410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15480..15783
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15784..15987
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15938..15958
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     16032..16051
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16032..16050
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggaggataa catggccatc
     3361 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     3421 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     3481 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     3541 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     3601 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     3661 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     3721 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     3781 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     3841 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     3901 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     3961 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     4021 gagctgtaca agggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg
     4081 gaggagaacc ctggacctat gagccagttc gacatcctgt gcaagacccc ccccaaggtg
     4141 ctggtgcggc agttcgtgga gagattcgag aggcccagcg gcgagaagat cgccagctgt
     4201 gccgccgagc tgacctacct gtgctggatg atcacccaca acggcaccgc catcaagagg
     4261 gccaccttca tgagctacaa caccatcatc agcaacagcc tgagcttcga catcgtgaac
     4321 aagagcctgc agttcaagta caagacccag aaggccacca tcctggaggc cagcctgaag
     4381 aagctgatcc ccgcctggga gttcaccatc atcccttaca acggccagaa gcaccagagc
     4441 gacatcaccg acatcgtgtc cagcctgcag ctgcagttcg agagcagcga ggaggccgac
     4501 aagggcaaca gccacagcaa gaagatgctg aaggccctgc tgtccgaggg cgagagcatc
     4561 tgggagatca ccgagaagat cctgaacagc ttcgagtaca ccagcaggtt caccaagacc
     4621 aagaccctgt accagttcct gttcctggcc acattcatca actgcggcag gttcagcgac
     4681 atcaagaacg tggaccccaa gagcttcaag ctggtgcaga acaagtacct gggcgtgatc
     4741 attcagtgcc tggtgaccga gaccaagaca agcgtgtcca ggcacatcta ctttttcagc
     4801 gccagaggca ggatcgaccc cctggtgtac ctggacgagt tcctgaggaa cagcgagccc
     4861 gtgctgaaga gagtgaacag gaccggcaac agcagcagca acaagcagga gtaccagctg
     4921 ctgaaggaca acctggtgcg cagctacaac aaggccctga agaagaacgc cccctacccc
     4981 atcttcgcta tcaagaacgg ccctaagagc cacatcggca ggcacctgat gaccagcttt
     5041 ctgagcatga agggcctgac cgagctgaca aacgtggtgg gcaactggag cgacaagagg
     5101 gcctccgccg tggccaggac cacctacacc caccagatca ccgccatccc cgaccactac
     5161 ttcgccctgg tgtccaggta ctacgcctac gaccccatca gcaaggagat gatcgccctg
     5221 aaggacgaga ccaaccccat cgaggagtgg cagcacatcg agcagctgaa gggcagcgcc
     5281 gagggcagca tcagataccc cgcctggaac ggcatcatca gccaggaggt gctggactac
     5341 ctgagcagct acatcaacag gcggatctga gctagcggcg cctagccggt catccttttg
     5401 acgattccag tcccgaggat aacctcctct cgggattggg gggaatcttt ggatccagta
     5461 gtcctccttg aactccgtcc aacagggcag attcaagagt cataagactt tcattaatca
     5521 tttcagttga tcagacatgg tcgtgtagat tctcttaata cgggagatct tctagcagtt
     5581 tcagtgacca acggtgcttt cattctccag gaactgatac caaaggttgt ggacaggcca
     5641 aggggtactt cggatgactc tgtgcttggg cacagaaaga ggtcgtagtg tgccccccga
     5701 tagcggactc gacatgaatc aactaagaaa ggcaatctgc ctcccatgat ggacataagc
     5761 aatagttcac aaccatcttg catctcagtg aagtgtacat aactattgag ggctgggtca
     5821 tctaagcatt tcagtcgaga aaaaaactgt agaccaaaag aacaactagc aacacttctc
     5881 atccagagac ccatatcaag atgctagatc cgggagaggt ttatgatgac cctattgatc
     5941 caattgagtc agaggctgaa cccagaggaa cccccactgt ccccaacatc ttgaggaact
     6001 ccgactacaa tctcaattct cctttgatag aggatcctgc caaactaatg ttagaatggt
     6061 tgaagacagg gaacagacct tatcggatga ctttgacaga caattgctcc aggtcttaca
     6121 aagttttgaa agattatttc aagaaagtag atttgggttc tctcaaagtg ggcggaactg
     6181 ctgcacagtc aatggtttct ctctggttgt gtggtgccca ctctgaatca aacaggagcc
     6241 ggagatgtat aaccgacttg gcccatttct attccaagtc atcccccata gagaagctat
     6301 tgaattgtac gctaggaaac agaggcctga gaatcccacc agagggggtg ttaaattgcc
     6361 tcgagagggt caattatgac aaggcatttg ggaggtatct ggccaacacg tattcctctt
     6421 acttgttttt ccatgtaatc accttataca tgaatgcctt agactgggaa gaggaaaaga
     6481 ccatcctggc attatggaaa gatataacct cagtggatac cgagaaggac ttggtcaaat
     6541 tcaaagatca aatatgggga ctgttgattg tgacaaagga ctttgtttac tctcagagtt
     6601 caaactgtct ttttgacaga aactacacac tgatgctaaa ggatcttttc ttgtctcgat
     6661 tcaactcctt aatgattctg ctttctcccc ctgagccccg atactcagat gacttaatat
     6721 ctcagctgtg ccagctatac atcgctgggg atcaagtctt gtccatgtgt gggaactccg
     6781 gctatgaagt catcaaaata ttggagccat atgtcgtgaa cagtttggtc cagagggcag
     6841 agaagtttag gcctctcatc caccccttgg gagactttcc tatgttcata aaagacaagg
     6901 tgaatcaact tgaagggact tttggtccca gtgcaaagag gttttttagg gttttagatc
     6961 aattcgacaa catacatgac ttagtatttg tgtatggctg ttacagacat tgggggcacc
     7021 cctatataga ttatcggaag ggtctgtcga aactatatga tcaagttcac attaagaaag
     7081 taatagataa gtcctaccag gagtgtttag caagtgactt ggccagaagg atcctcagat
     7141 ggggatttga caagtactcc aaatggtatc tagattcgag attccttgcc cgagaccacc
     7201 ccttgactcc ttatgtcaag acccaaacat ggccacccaa acatatagta gacttggtgg
     7261 gggacacatg gcataagctc ccgatcacgc agatctttga aattcctgaa tcaatggacc
     7321 cgtcagagat actggatgac aaatcacatt ctttcaccag aacaaggtta gcttcttggc
     7381 tgtcagagaa ccgagggggg cctgttccta gcgagaaggt cattatcacg gccctgtcta
     7441 agccacctgt caatccccga gagtttttga aatctataga cctcggagga ttgccagatg
     7501 aggatttgat aattggcctc aaaccaaagg aacgggagtt gaagatcgag ggccgattct
     7561 ttgctctaat gtcatggaat ctaagattat attttgtcat caccgaaaag ctcttggcca
     7621 actacatttt gccacttttt gacgcactga ctatgacaga caacctgaac aaggtgttca
     7681 aaaagttgat cgacagggtc accgggcaag ggcttttgga ctattctagg gtcacatacg
     7741 catttcacct ggactatgag aaatggaaca atcatcaaag attggagtca acagaggatg
     7801 tattctctgt cctagatcag gtgtttggat tgaagagggt gttttctaga acacacgagt
     7861 tttttcagaa gtcctggatc tattattcag acagatcaga cctcattggg ttatgggagg
     7921 accaaatata ttgcttggat atgtctaacg gcccaacctg ctggaatggc caagatggcg
     7981 ggctagaggg cttgcggcag aagggctgga gtctagtcag tttattaatg atagatagag
     8041 aatctcaaac caggaacaca agaaccaaga tactagctca aggagacaac caggttctgt
     8101 gtcctacata catgttgtca ccgggattgt ctcaagaggg gcttctctat gagttagaga
     8161 gcatatcaag gaatgcactc tcaatatacc gagctatcga ggaaggagca tctaagctgg
     8221 ggctgatcat caagaaggaa gagaccatgt gtagttatga ctttctcata tatgggaaga
     8281 cccccttatt tcgaggcaac atattggtgc ctgaatccaa aagatgggcc cgagtctctt
     8341 gcatctctaa cgaccaaata gtcaacctcg ccaatataat gtcgacagta tccaccaatg
     8401 cgctgacagt ggcacaacac tctcaatctc tgatcaaacc tatgagggat tttctgctca
     8461 tgtcagtaca ggcagttttc cactacctgt tgtttagccc aatcctaaaa ggcagagttt
     8521 ataagattct gagtgctgaa ggggagagct ttctcctagc catgtcgcgg ataatctacc
     8581 tagatccttc tttgggaggg gtgtctggaa tgtctctcgg gaggttccat atacgtcagt
     8641 tctcagaccc tgtctctgaa gggttgtcat tctggagaga gatctggtta ggctctcatg
     8701 agtcctggat tcacgcgttg tgtcaggagg ccgggaaccc cgatcttgga gagagaacac
     8761 tcgagagctt cactcgcctt ttagaagatc ctactacctt aaatatcaaa ggaggggcca
     8821 gccctaccat tctactcaag gatgctatca gaaaggctct gtacgacgag gtggacaagg
     8881 tggaaaattc agagtttcga gaggcaatcc tgctgtccaa gacccataga gataacttta
     8941 tactcttttt aaaatctgtt gagcctctgt tccctcgatt tctcagtgag ctcttcagtt
     9001 cgtctttctt gggaataccg gagtcaatca ttggactgat acaaaactcc cggacaataa
     9061 gaaggcagtt tagaaagagt ctctcaagaa ctttagaaga gtccttctac aactcagaga
     9121 tccacgggat taatcggata acccagacac ctcaaagggt cggaagggtg tggccttgct
     9181 cttcagagag ggcggatcta cttagggaga tctcttgggg gaggaaagtg gtaggcacga
     9241 cagttcctca cccttccgag atgttggagt tgtttcccaa atcctccatt tcctgcactt
     9301 gtggagcaac agggggaggc aatcctagag tctctgtatc agtactcccg tccttcgatc
     9361 agtcattttt ctcacggggc cccctaaagg gatacttggg ctcgtccacc tctatgtcaa
     9421 cccagctatt ccatgcatgg gaaaaagtca ctaatgttca tgtggtgaaa agggctctat
     9481 cgttaaaaga atctataaac tggttcatca ataggaattc caatttggct caaactctaa
     9541 ttggaaacat catgtctctg acaggccctg acttccctct agaagaggcc cctgttttca
     9601 aacggacagg gtcagccttg cataggttca agtctgccag atacagcgaa ggagggtatt
     9661 cttctgtttg ccctaacctt ctctcccata tctctgttag tacagacact atgtctgatt
     9721 tgacccaaaa cgggaagaac tatgatttca tgtttcagcc attgatgctt tatgcgcaaa
     9781 catggacatc ggaactggta cagagggata caagacttag agactccacg tttcactggc
     9841 accttcggtg caacagatgt gtgaggccca ttgatgatat aacactggaa acttctcaga
     9901 tcttcgagtt cccggatgtg tcaaaaagga tatccaggat ggtttctgga gctgtccctc
     9961 aatttcagaa gcttcctgat atccgtctaa gaccaggtga ttttgaatcc ctaagtggta
    10021 gagaaaagtc tcgccatata gggtcagctc aggggctctt atactcaatc ttagtagcaa
    10081 ttcacgactc aggatacaat gatgggacca tcttccctgt caacatatac ggcaaagttt
    10141 cccccagaga ctatttgaga gggcttgcaa gagggatctt gatagggtcc tcgatttgct
    10201 tcttgacacg aatgacaaat attaacatta acagacctct tgaattgatc tcaggggtaa
    10261 tttcctatat tctcctgagg ctggataatc atccctctct gtatataatg ctcagagaac
    10321 cgtctcttag aggagaaata ttctctatcc ctcagaaaat ccccgccgct tacccaacca
    10381 ctatgagaga aggcaacaga tcgattttgt gttacctcca acacgtgcta cgctatgagc
    10441 gagaggcaat cacggcgtcc ccggagaatg actggctgtg gatcttctca gacttcagaa
    10501 gtgtgaaaat gacgtacttg accctcatta cttaccagtc tcacctccta ctccagaggg
    10561 ttgagagaaa cttgtctaag agtatgagag ctactctgcg acaaatgggt tccttaatga
    10621 ggcaagtgct gggtgggcac ggagaagata ccttggagtc agacgatgac attcaacgat
    10681 tactaaaaga ctctttgcga aagacaaggt gggtggatca agaggtgcgc catgcagcta
    10741 gaaccatgaa tggagattac agccccgaca agaaagtatc ccgtaaggta ggatgttcag
    10801 aatgggtctg ctctgctcaa cagattgccg tctccacctc agccaacccg gcccctgtct
    10861 cagagcttga cattagggcc ctctctaagc ggtttcaaaa ccccttgatc tcgggcctga
    10921 gagtggttca gtgggcaacc ggtgcccatt ataagcttaa gcctattcta gatgatctaa
    10981 atgttttccc atctctctgt cttgtaattg gagacgggtc agggggaata tcaagggcag
    11041 ttctcaacat gtttccagat tctaagcttg tgttcaacag cctattggag gtgaatgatc
    11101 tgatggcttc cggaacacat ccactgcctc cttcagcaat catgagtgga ggagatgaca
    11161 tcatctccag agtgatagac tttgactcaa tatgggagaa accgtccgac ctgaggaact
    11221 cggccacctg gagatacttc cagtcggttc aaaaacaggt caacatgtcg tatgacctca
    11281 ttatttgtga tgcagaagtt actgatattg catctatcaa ccggataact ctgttgatgt
    11341 ctgatttcgc attgtctata gatggaccac tttatctggt cttcaaaact tacgggacta
    11401 tgctagtaaa cccggactat aaagctattc aacatctgtc aagagcgttc ccttcggtca
    11461 cagggtttgt aacccaagta acttcatcct tttcttctga gctatacctc cggttctcta
    11521 aacgaggaaa gtttttcagg gacgccgagt acttgacctc ttccaccctt cgagagatga
    11581 gccttgtgtt gttcaattgc agcagcccca aaagtgagat gcagagagct cgttccttaa
    11641 actatcaaga cctggtaagg ggatttcctg aagagatcat atcaaatcct tacaacgaaa
    11701 tgatcataac tctgattgac aatgatgtag agtccttcct agtccacaag atggtggatg
    11761 atcttgagct acagagggga actctgtcta aagtggctat cattatatcc atcatgatcg
    11821 ttttttccaa tagagtcttc aacatttcca aacctttgac tgaccccttg ttctatccac
    11881 cgtctgatcc taaaatcctg aggcacttca acatatgttg cagtactatg atgtatctat
    11941 ctaccgcttt aggcgacgtc cctaacttcg caagacttca tgacctgtat aacagaccta
    12001 taacttgtta cttcagaaaa caagttattc gagggaatat ttatctatct tggagttggt
    12061 ccgatgatac cccagtgttc aagagagtag cctgtaattc tagcttgagt ctgtcatctc
    12121 actggatcag gttgatctac aagatagtga agactaccag actcattggc agcataaaag
    12181 acctatcagg agaggtagaa cgacatcttc atgggtataa cagatggatc accctcgagg
    12241 atatccgatc tagatcatcc ctactagatt acagttgttt gtaagccgga tattaccgaa
    12301 agcctgtgca tgctaaaatt cttgtatgat gcatcttgaa aaaaacaaga tcttgaatcc
    12361 ggacctctgg ttgtttgatt gttttttcca tctttattgt ttttttgtta agcgtgggtc
    12421 ggcatggcat ctccacctcc tcgcggtccg acctgggcat ccgaaggagg acgcacgtcc
    12481 actcggatgg ctaagggagg ggcggggatc cggctgctaa caaagcccga aaggaagctg
    12541 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
    12601 tcttgagggg ttttttgctg aaagtcgcgc ttggcgtaat catggtcata gctgtttcct
    12661 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    12721 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    12781 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    12841 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    12901 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    12961 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    13021 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    13081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    13141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    13201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    13261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    13321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    13381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    13441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    13501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    13561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    13621 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    13681 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    13741 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    13801 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    13861 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    13921 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    13981 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    14041 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    14101 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    14161 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    14221 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    14281 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    14341 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    14401 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    14461 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    14521 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    14581 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    14641 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    14701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    14761 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
    14821 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    14881 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    14941 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    15001 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    15061 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    15121 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    15181 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    15241 acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    15301 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    15361 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcct
    15421 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
    15481 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
    15541 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
    15601 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
    15661 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
    15721 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
    15781 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
    15841 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
    15901 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
    15961 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
    16021 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagat taagcgtctg
    16081 atgagtccgt gaggacgaaa cccggcgtac cgggtc
//
