LOCUS       sequence-135704-       15693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses APEX2 fused to Venus for transsynaptic tracing and the EM 
            analysis of infected neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15693)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 15693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-APEX2-Venus
FEATURES             Location/Qualifiers
     source          1..15693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3328..3351
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3352..4098
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             4105..4131
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     regulatory      4147..4156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4153..4155,4156..4158,4159..4869)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4198..4219)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4459..4478)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4806..4827
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7122..7133
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8925..8936)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12133..12151)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12147..12194
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12222..12238)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12222..12238)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12235..12257)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12246..12262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12270..12276,12277..12294,12295..12300))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12315..12336
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12453..12470)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12624..13212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12704..12723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13383..14174,14175..14243))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14006..14025
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14244..14348)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14375..14830
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14462..14481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14672..14693
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14956..14978
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14970..14987
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14971..14987
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15057..15360
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15361..15564
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15515..15535
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     15609..15628
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15609..15627
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggac tacaaggatg acgacgataa gggaaagtct
     3361 tacccaactg tgagtgctga ttaccaggac gccgttgaga aggcgaagaa gaagctcaga
     3421 ggcttcatcg ctgagaagag atgcgctcct ctaatgctcc gtttggcatt ccactctgct
     3481 ggaacctttg acaagggcac gaagaccggt ggacccttcg gaaccatcaa gcaccctgcc
     3541 gaactggctc acagcgctaa caacggtctt gacatcgctg ttaggctttt ggagccactc
     3601 aaggcggagt tccctatttt gagctacgcc gatttctacc agttggctgg cgttgttgcc
     3661 gttgaggtca cgggtggacc taaggttcca ttccaccctg gaagagagga caagcctgag
     3721 ccaccaccag agggtcgctt gcccgatccc actaagggtt ctgaccattt gagagatgtg
     3781 tttggcaaag ctatggggct tactgaccaa gatatcgttg ctctatctgg gggtcacact
     3841 attggagctg cacacaagga gcgttctgga tttgagggtc cctggacctc taatcctctt
     3901 attttcgaca actcatactt cacggagttg ttgagtggtg agaaggaagg tctccttcag
     3961 ctaccttctg acaaggctct tttgtctgac cctgtattcc gccctctcgt tgacaaatat
     4021 gcagcggacg aagatgcctt ctttgctgat tacgctgagg ctcaccaaaa gctttccgag
     4081 cttgggtttg ctgatgccct gcagctgcct cccctggagc gcctgaccct ggacggaatt
     4141 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     4201 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     4261 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
     4321 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
     4381 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4441 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4501 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4561 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     4621 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     4681 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4741 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     4801 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4861 ctgtacaagt ccggactcag atctaagctg aaccctcctg atgagagtgg ccccggctgc
     4921 atgagctgca agtgtgtgct ctcctgagct agcggcgcct agccggtcat ccttttgacg
     4981 attccagtcc cgaggataac ctcctctcgg gattgggggg aatctttgga tccagtagtc
     5041 ctccttgaac tccgtccaac agggcagatt caagagtcat aagactttca ttaatcattt
     5101 cagttgatca gacatggtcg tgtagattct cttaatacgg gagatcttct agcagtttca
     5161 gtgaccaacg gtgctttcat tctccaggaa ctgataccaa aggttgtgga caggccaagg
     5221 ggtacttcgg atgactctgt gcttgggcac agaaagaggt cgtagtgtgc cccccgatag
     5281 cggactcgac atgaatcaac taagaaaggc aatctgcctc ccatgatgga cataagcaat
     5341 agttcacaac catcttgcat ctcagtgaag tgtacataac tattgagggc tgggtcatct
     5401 aagcatttca gtcgagaaaa aaactgtaga ccaaaagaac aactagcaac acttctcatc
     5461 cagagaccca tatcaagatg ctagatccgg gagaggttta tgatgaccct attgatccaa
     5521 ttgagtcaga ggctgaaccc agaggaaccc ccactgtccc caacatcttg aggaactccg
     5581 actacaatct caattctcct ttgatagagg atcctgccaa actaatgtta gaatggttga
     5641 agacagggaa cagaccttat cggatgactt tgacagacaa ttgctccagg tcttacaaag
     5701 ttttgaaaga ttatttcaag aaagtagatt tgggttctct caaagtgggc ggaactgctg
     5761 cacagtcaat ggtttctctc tggttgtgtg gtgcccactc tgaatcaaac aggagccgga
     5821 gatgtataac cgacttggcc catttctatt ccaagtcatc ccccatagag aagctattga
     5881 attgtacgct aggaaacaga ggcctgagaa tcccaccaga gggggtgtta aattgcctcg
     5941 agagggtcaa ttatgacaag gcatttggga ggtatctggc caacacgtat tcctcttact
     6001 tgtttttcca tgtaatcacc ttatacatga atgccttaga ctgggaagag gaaaagacca
     6061 tcctggcatt atggaaagat ataacctcag tggataccga gaaggacttg gtcaaattca
     6121 aagatcaaat atggggactg ttgattgtga caaaggactt tgtttactct cagagttcaa
     6181 actgtctttt tgacagaaac tacacactga tgctaaagga tcttttcttg tctcgattca
     6241 actccttaat gattctgctt tctccccctg agccccgata ctcagatgac ttaatatctc
     6301 agctgtgcca gctatacatc gctggggatc aagtcttgtc catgtgtggg aactccggct
     6361 atgaagtcat caaaatattg gagccatatg tcgtgaacag tttggtccag agggcagaga
     6421 agtttaggcc tctcatccac cccttgggag actttcctat gttcataaaa gacaaggtga
     6481 atcaacttga agggactttt ggtcccagtg caaagaggtt ttttagggtt ttagatcaat
     6541 tcgacaacat acatgactta gtatttgtgt atggctgtta cagacattgg gggcacccct
     6601 atatagatta tcggaagggt ctgtcgaaac tatatgatca agttcacatt aagaaagtaa
     6661 tagataagtc ctaccaggag tgtttagcaa gtgacttggc cagaaggatc ctcagatggg
     6721 gatttgacaa gtactccaaa tggtatctag attcgagatt ccttgcccga gaccacccct
     6781 tgactcctta tgtcaagacc caaacatggc cacccaaaca tatagtagac ttggtggggg
     6841 acacatggca taagctcccg atcacgcaga tctttgaaat tcctgaatca atggacccgt
     6901 cagagatact ggatgacaaa tcacattctt tcaccagaac aaggttagct tcttggctgt
     6961 cagagaaccg aggggggcct gttcctagcg agaaggtcat tatcacggcc ctgtctaagc
     7021 cacctgtcaa tccccgagag tttttgaaat ctatagacct cggaggattg ccagatgagg
     7081 atttgataat tggcctcaaa ccaaaggaac gggagttgaa gatcgagggc cgattctttg
     7141 ctctaatgtc atggaatcta agattatatt ttgtcatcac cgaaaagctc ttggccaact
     7201 acattttgcc actttttgac gcactgacta tgacagacaa cctgaacaag gtgttcaaaa
     7261 agttgatcga cagggtcacc gggcaagggc ttttggacta ttctagggtc acatacgcat
     7321 ttcacctgga ctatgagaaa tggaacaatc atcaaagatt ggagtcaaca gaggatgtat
     7381 tctctgtcct agatcaggtg tttggattga agagggtgtt ttctagaaca cacgagtttt
     7441 ttcagaagtc ctggatctat tattcagaca gatcagacct cattgggtta tgggaggacc
     7501 aaatatattg cttggatatg tctaacggcc caacctgctg gaatggccaa gatggcgggc
     7561 tagagggctt gcggcagaag ggctggagtc tagtcagttt attaatgata gatagagaat
     7621 ctcaaaccag gaacacaaga accaagatac tagctcaagg agacaaccag gttctgtgtc
     7681 ctacatacat gttgtcaccg ggattgtctc aagaggggct tctctatgag ttagagagca
     7741 tatcaaggaa tgcactctca atataccgag ctatcgagga aggagcatct aagctggggc
     7801 tgatcatcaa gaaggaagag accatgtgta gttatgactt tctcatatat gggaagaccc
     7861 ccttatttcg aggcaacata ttggtgcctg aatccaaaag atgggcccga gtctcttgca
     7921 tctctaacga ccaaatagtc aacctcgcca atataatgtc gacagtatcc accaatgcgc
     7981 tgacagtggc acaacactct caatctctga tcaaacctat gagggatttt ctgctcatgt
     8041 cagtacaggc agttttccac tacctgttgt ttagcccaat cctaaaaggc agagtttata
     8101 agattctgag tgctgaaggg gagagctttc tcctagccat gtcgcggata atctacctag
     8161 atccttcttt gggaggggtg tctggaatgt ctctcgggag gttccatata cgtcagttct
     8221 cagaccctgt ctctgaaggg ttgtcattct ggagagagat ctggttaggc tctcatgagt
     8281 cctggattca cgcgttgtgt caggaggccg ggaaccccga tcttggagag agaacactcg
     8341 agagcttcac tcgcctttta gaagatccta ctaccttaaa tatcaaagga ggggccagcc
     8401 ctaccattct actcaaggat gctatcagaa aggctctgta cgacgaggtg gacaaggtgg
     8461 aaaattcaga gtttcgagag gcaatcctgc tgtccaagac ccatagagat aactttatac
     8521 tctttttaaa atctgttgag cctctgttcc ctcgatttct cagtgagctc ttcagttcgt
     8581 ctttcttggg aataccggag tcaatcattg gactgataca aaactcccgg acaataagaa
     8641 ggcagtttag aaagagtctc tcaagaactt tagaagagtc cttctacaac tcagagatcc
     8701 acgggattaa tcggataacc cagacacctc aaagggtcgg aagggtgtgg ccttgctctt
     8761 cagagagggc ggatctactt agggagatct cttgggggag gaaagtggta ggcacgacag
     8821 ttcctcaccc ttccgagatg ttggagttgt ttcccaaatc ctccatttcc tgcacttgtg
     8881 gagcaacagg gggaggcaat cctagagtct ctgtatcagt actcccgtcc ttcgatcagt
     8941 catttttctc acggggcccc ctaaagggat acttgggctc gtccacctct atgtcaaccc
     9001 agctattcca tgcatgggaa aaagtcacta atgttcatgt ggtgaaaagg gctctatcgt
     9061 taaaagaatc tataaactgg ttcatcaata ggaattccaa tttggctcaa actctaattg
     9121 gaaacatcat gtctctgaca ggccctgact tccctctaga agaggcccct gttttcaaac
     9181 ggacagggtc agccttgcat aggttcaagt ctgccagata cagcgaagga gggtattctt
     9241 ctgtttgccc taaccttctc tcccatatct ctgttagtac agacactatg tctgatttga
     9301 cccaaaacgg gaagaactat gatttcatgt ttcagccatt gatgctttat gcgcaaacat
     9361 ggacatcgga actggtacag agggatacaa gacttagaga ctccacgttt cactggcacc
     9421 ttcggtgcaa cagatgtgtg aggcccattg atgatataac actggaaact tctcagatct
     9481 tcgagttccc ggatgtgtca aaaaggatat ccaggatggt ttctggagct gtccctcaat
     9541 ttcagaagct tcctgatatc cgtctaagac caggtgattt tgaatcccta agtggtagag
     9601 aaaagtctcg ccatataggg tcagctcagg ggctcttata ctcaatctta gtagcaattc
     9661 acgactcagg atacaatgat gggaccatct tccctgtcaa catatacggc aaagtttccc
     9721 ccagagacta tttgagaggg cttgcaagag ggatcttgat agggtcctcg atttgcttct
     9781 tgacacgaat gacaaatatt aacattaaca gacctcttga attgatctca ggggtaattt
     9841 cctatattct cctgaggctg gataatcatc cctctctgta tataatgctc agagaaccgt
     9901 ctcttagagg agaaatattc tctatccctc agaaaatccc cgccgcttac ccaaccacta
     9961 tgagagaagg caacagatcg attttgtgtt acctccaaca cgtgctacgc tatgagcgag
    10021 aggcaatcac ggcgtccccg gagaatgact ggctgtggat cttctcagac ttcagaagtg
    10081 tgaaaatgac gtacttgacc ctcattactt accagtctca cctcctactc cagagggttg
    10141 agagaaactt gtctaagagt atgagagcta ctctgcgaca aatgggttcc ttaatgaggc
    10201 aagtgctggg tgggcacgga gaagatacct tggagtcaga cgatgacatt caacgattac
    10261 taaaagactc tttgcgaaag acaaggtggg tggatcaaga ggtgcgccat gcagctagaa
    10321 ccatgaatgg agattacagc cccgacaaga aagtatcccg taaggtagga tgttcagaat
    10381 gggtctgctc tgctcaacag attgccgtct ccacctcagc caacccggcc cctgtctcag
    10441 agcttgacat tagggccctc tctaagcggt ttcaaaaccc cttgatctcg ggcctgagag
    10501 tggttcagtg ggcaaccggt gcccattata agcttaagcc tattctagat gatctaaatg
    10561 ttttcccatc tctctgtctt gtaattggag acgggtcagg gggaatatca agggcagttc
    10621 tcaacatgtt tccagattct aagcttgtgt tcaacagcct attggaggtg aatgatctga
    10681 tggcttccgg aacacatcca ctgcctcctt cagcaatcat gagtggagga gatgacatca
    10741 tctccagagt gatagacttt gactcaatat gggagaaacc gtccgacctg aggaactcgg
    10801 ccacctggag atacttccag tcggttcaaa aacaggtcaa catgtcgtat gacctcatta
    10861 tttgtgatgc agaagttact gatattgcat ctatcaaccg gataactctg ttgatgtctg
    10921 atttcgcatt gtctatagat ggaccacttt atctggtctt caaaacttac gggactatgc
    10981 tagtaaaccc ggactataaa gctattcaac atctgtcaag agcgttccct tcggtcacag
    11041 ggtttgtaac ccaagtaact tcatcctttt cttctgagct atacctccgg ttctctaaac
    11101 gaggaaagtt tttcagggac gccgagtact tgacctcttc cacccttcga gagatgagcc
    11161 ttgtgttgtt caattgcagc agccccaaaa gtgagatgca gagagctcgt tccttaaact
    11221 atcaagacct ggtaagggga tttcctgaag agatcatatc aaatccttac aacgaaatga
    11281 tcataactct gattgacaat gatgtagagt ccttcctagt ccacaagatg gtggatgatc
    11341 ttgagctaca gaggggaact ctgtctaaag tggctatcat tatatccatc atgatcgttt
    11401 tttccaatag agtcttcaac atttccaaac ctttgactga ccccttgttc tatccaccgt
    11461 ctgatcctaa aatcctgagg cacttcaaca tatgttgcag tactatgatg tatctatcta
    11521 ccgctttagg cgacgtccct aacttcgcaa gacttcatga cctgtataac agacctataa
    11581 cttgttactt cagaaaacaa gttattcgag ggaatattta tctatcttgg agttggtccg
    11641 atgatacccc agtgttcaag agagtagcct gtaattctag cttgagtctg tcatctcact
    11701 ggatcaggtt gatctacaag atagtgaaga ctaccagact cattggcagc ataaaagacc
    11761 tatcaggaga ggtagaacga catcttcatg ggtataacag atggatcacc ctcgaggata
    11821 tccgatctag atcatcccta ctagattaca gttgtttgta agccggatat taccgaaagc
    11881 ctgtgcatgc taaaattctt gtatgatgca tcttgaaaaa aacaagatct tgaatccgga
    11941 cctctggttg tttgattgtt ttttccatct ttattgtttt tttgttaagc gtgggtcggc
    12001 atggcatctc cacctcctcg cggtccgacc tgggcatccg aaggaggacg cacgtccact
    12061 cggatggcta agggaggggc ggggatccgg ctgctaacaa agcccgaaag gaagctgagt
    12121 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
    12181 tgaggggttt tttgctgaaa gtcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
    12241 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    12301 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    12361 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    12421 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    12481 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    12541 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    12601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    12661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    12721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    12781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    12841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    12901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    12961 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    13021 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    13081 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    13141 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    13201 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    13261 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    13321 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    13381 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    13441 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    13501 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    13561 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    13621 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    13681 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    13741 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    13801 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    13861 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    13921 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    13981 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    14041 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    14101 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    14161 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    14221 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    14281 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    14341 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
    14401 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    14461 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    14521 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    14581 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    14641 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    14701 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    14761 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    14821 cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    14881 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    14941 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcctagt
    15001 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
    15061 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
    15121 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
    15181 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
    15241 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
    15301 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
    15361 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
    15421 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
    15481 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
    15541 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
    15601 atcgaaatta atacgactca ctatagggag acccaagctg gctagattaa gcgtctgatg
    15661 agtccgtgag gacgaaaccc ggcgtaccgg gtc
//
