LOCUS       sequence-135705-       15321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GCaMP6f for calcium imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15321)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 15321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..15321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3340..3396
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     3609..3630
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3736..3757)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3997..4016)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             6750..6761
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8553..8564)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11761..11779)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11775..11822
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11850..11866)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11850..11866)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11863..11885)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11874..11890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11898..11904,11905..11922,11923..11928))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11943..11964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12081..12098)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12252..12840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12332..12351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13011..13802,13803..13871))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13634..13653
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13872..13976)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14003..14458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14090..14109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14300..14321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14584..14606
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14598..14615
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14599..14615
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        14685..14988
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14989..15192
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15143..15163
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     15237..15256
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15237..15255
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtc gactcatcac gtcgtaagtg gaataagaca
     3361 ggtcacgcag tcagagctat aggtcggctg agctcactcg agaacgtcta tatcaaggcc
     3421 gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc
     3481 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3541 ctgcccgaca accactacct gagcgtgcag tccaaacttt cgaaagaccc caacgagaag
     3601 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3661 gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc
     3721 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     3781 tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     3841 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     3901 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     3961 gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4021 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4081 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacctgcc ggaccaactg
     4141 actgaagagc agatcgcaga atttaaagag gaattctccc tatttgacaa ggacggggat
     4201 gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca gaaccccaca
     4261 gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac
     4321 ttccctgagt tcctgacaat gatggcaaga aaaatgaaat acagggacac ggaagaagaa
     4381 attagagaag cgttcggtgt gtttgataag gatggcaatg gctacatcag tgcagcagag
     4441 cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt tgatgaaatg
     4501 atcagggaag cagacatcga tggggatggt caggtaaact acgaagagtt tgtacaaatg
     4561 atgacagcga agtgagctag cggcgcctag ccggtcatcc ttttgacgat tccagtcccg
     4621 aggataacct cctctcggga ttggggggaa tctttggatc cagtagtcct ccttgaactc
     4681 cgtccaacag ggcagattca agagtcataa gactttcatt aatcatttca gttgatcaga
     4741 catggtcgtg tagattctct taatacggga gatcttctag cagtttcagt gaccaacggt
     4801 gctttcattc tccaggaact gataccaaag gttgtggaca ggccaagggg tacttcggat
     4861 gactctgtgc ttgggcacag aaagaggtcg tagtgtgccc cccgatagcg gactcgacat
     4921 gaatcaacta agaaaggcaa tctgcctccc atgatggaca taagcaatag ttcacaacca
     4981 tcttgcatct cagtgaagtg tacataacta ttgagggctg ggtcatctaa gcatttcagt
     5041 cgagaaaaaa actgtagacc aaaagaacaa ctagcaacac ttctcatcca gagacccata
     5101 tcaagatgct agatccggga gaggtttatg atgaccctat tgatccaatt gagtcagagg
     5161 ctgaacccag aggaaccccc actgtcccca acatcttgag gaactccgac tacaatctca
     5221 attctccttt gatagaggat cctgccaaac taatgttaga atggttgaag acagggaaca
     5281 gaccttatcg gatgactttg acagacaatt gctccaggtc ttacaaagtt ttgaaagatt
     5341 atttcaagaa agtagatttg ggttctctca aagtgggcgg aactgctgca cagtcaatgg
     5401 tttctctctg gttgtgtggt gcccactctg aatcaaacag gagccggaga tgtataaccg
     5461 acttggccca tttctattcc aagtcatccc ccatagagaa gctattgaat tgtacgctag
     5521 gaaacagagg cctgagaatc ccaccagagg gggtgttaaa ttgcctcgag agggtcaatt
     5581 atgacaaggc atttgggagg tatctggcca acacgtattc ctcttacttg tttttccatg
     5641 taatcacctt atacatgaat gccttagact gggaagagga aaagaccatc ctggcattat
     5701 ggaaagatat aacctcagtg gataccgaga aggacttggt caaattcaaa gatcaaatat
     5761 ggggactgtt gattgtgaca aaggactttg tttactctca gagttcaaac tgtctttttg
     5821 acagaaacta cacactgatg ctaaaggatc ttttcttgtc tcgattcaac tccttaatga
     5881 ttctgctttc tccccctgag ccccgatact cagatgactt aatatctcag ctgtgccagc
     5941 tatacatcgc tggggatcaa gtcttgtcca tgtgtgggaa ctccggctat gaagtcatca
     6001 aaatattgga gccatatgtc gtgaacagtt tggtccagag ggcagagaag tttaggcctc
     6061 tcatccaccc cttgggagac tttcctatgt tcataaaaga caaggtgaat caacttgaag
     6121 ggacttttgg tcccagtgca aagaggtttt ttagggtttt agatcaattc gacaacatac
     6181 atgacttagt atttgtgtat ggctgttaca gacattgggg gcacccctat atagattatc
     6241 ggaagggtct gtcgaaacta tatgatcaag ttcacattaa gaaagtaata gataagtcct
     6301 accaggagtg tttagcaagt gacttggcca gaaggatcct cagatgggga tttgacaagt
     6361 actccaaatg gtatctagat tcgagattcc ttgcccgaga ccaccccttg actccttatg
     6421 tcaagaccca aacatggcca cccaaacata tagtagactt ggtgggggac acatggcata
     6481 agctcccgat cacgcagatc tttgaaattc ctgaatcaat ggacccgtca gagatactgg
     6541 atgacaaatc acattctttc accagaacaa ggttagcttc ttggctgtca gagaaccgag
     6601 gggggcctgt tcctagcgag aaggtcatta tcacggccct gtctaagcca cctgtcaatc
     6661 cccgagagtt tttgaaatct atagacctcg gaggattgcc agatgaggat ttgataattg
     6721 gcctcaaacc aaaggaacgg gagttgaaga tcgagggccg attctttgct ctaatgtcat
     6781 ggaatctaag attatatttt gtcatcaccg aaaagctctt ggccaactac attttgccac
     6841 tttttgacgc actgactatg acagacaacc tgaacaaggt gttcaaaaag ttgatcgaca
     6901 gggtcaccgg gcaagggctt ttggactatt ctagggtcac atacgcattt cacctggact
     6961 atgagaaatg gaacaatcat caaagattgg agtcaacaga ggatgtattc tctgtcctag
     7021 atcaggtgtt tggattgaag agggtgtttt ctagaacaca cgagtttttt cagaagtcct
     7081 ggatctatta ttcagacaga tcagacctca ttgggttatg ggaggaccaa atatattgct
     7141 tggatatgtc taacggccca acctgctgga atggccaaga tggcgggcta gagggcttgc
     7201 ggcagaaggg ctggagtcta gtcagtttat taatgataga tagagaatct caaaccagga
     7261 acacaagaac caagatacta gctcaaggag acaaccaggt tctgtgtcct acatacatgt
     7321 tgtcaccggg attgtctcaa gaggggcttc tctatgagtt agagagcata tcaaggaatg
     7381 cactctcaat ataccgagct atcgaggaag gagcatctaa gctggggctg atcatcaaga
     7441 aggaagagac catgtgtagt tatgactttc tcatatatgg gaagaccccc ttatttcgag
     7501 gcaacatatt ggtgcctgaa tccaaaagat gggcccgagt ctcttgcatc tctaacgacc
     7561 aaatagtcaa cctcgccaat ataatgtcga cagtatccac caatgcgctg acagtggcac
     7621 aacactctca atctctgatc aaacctatga gggattttct gctcatgtca gtacaggcag
     7681 ttttccacta cctgttgttt agcccaatcc taaaaggcag agtttataag attctgagtg
     7741 ctgaagggga gagctttctc ctagccatgt cgcggataat ctacctagat ccttctttgg
     7801 gaggggtgtc tggaatgtct ctcgggaggt tccatatacg tcagttctca gaccctgtct
     7861 ctgaagggtt gtcattctgg agagagatct ggttaggctc tcatgagtcc tggattcacg
     7921 cgttgtgtca ggaggccggg aaccccgatc ttggagagag aacactcgag agcttcactc
     7981 gccttttaga agatcctact accttaaata tcaaaggagg ggccagccct accattctac
     8041 tcaaggatgc tatcagaaag gctctgtacg acgaggtgga caaggtggaa aattcagagt
     8101 ttcgagaggc aatcctgctg tccaagaccc atagagataa ctttatactc tttttaaaat
     8161 ctgttgagcc tctgttccct cgatttctca gtgagctctt cagttcgtct ttcttgggaa
     8221 taccggagtc aatcattgga ctgatacaaa actcccggac aataagaagg cagtttagaa
     8281 agagtctctc aagaacttta gaagagtcct tctacaactc agagatccac gggattaatc
     8341 ggataaccca gacacctcaa agggtcggaa gggtgtggcc ttgctcttca gagagggcgg
     8401 atctacttag ggagatctct tgggggagga aagtggtagg cacgacagtt cctcaccctt
     8461 ccgagatgtt ggagttgttt cccaaatcct ccatttcctg cacttgtgga gcaacagggg
     8521 gaggcaatcc tagagtctct gtatcagtac tcccgtcctt cgatcagtca tttttctcac
     8581 ggggccccct aaagggatac ttgggctcgt ccacctctat gtcaacccag ctattccatg
     8641 catgggaaaa agtcactaat gttcatgtgg tgaaaagggc tctatcgtta aaagaatcta
     8701 taaactggtt catcaatagg aattccaatt tggctcaaac tctaattgga aacatcatgt
     8761 ctctgacagg ccctgacttc cctctagaag aggcccctgt tttcaaacgg acagggtcag
     8821 ccttgcatag gttcaagtct gccagataca gcgaaggagg gtattcttct gtttgcccta
     8881 accttctctc ccatatctct gttagtacag acactatgtc tgatttgacc caaaacggga
     8941 agaactatga tttcatgttt cagccattga tgctttatgc gcaaacatgg acatcggaac
     9001 tggtacagag ggatacaaga cttagagact ccacgtttca ctggcacctt cggtgcaaca
     9061 gatgtgtgag gcccattgat gatataacac tggaaacttc tcagatcttc gagttcccgg
     9121 atgtgtcaaa aaggatatcc aggatggttt ctggagctgt ccctcaattt cagaagcttc
     9181 ctgatatccg tctaagacca ggtgattttg aatccctaag tggtagagaa aagtctcgcc
     9241 atatagggtc agctcagggg ctcttatact caatcttagt agcaattcac gactcaggat
     9301 acaatgatgg gaccatcttc cctgtcaaca tatacggcaa agtttccccc agagactatt
     9361 tgagagggct tgcaagaggg atcttgatag ggtcctcgat ttgcttcttg acacgaatga
     9421 caaatattaa cattaacaga cctcttgaat tgatctcagg ggtaatttcc tatattctcc
     9481 tgaggctgga taatcatccc tctctgtata taatgctcag agaaccgtct cttagaggag
     9541 aaatattctc tatccctcag aaaatccccg ccgcttaccc aaccactatg agagaaggca
     9601 acagatcgat tttgtgttac ctccaacacg tgctacgcta tgagcgagag gcaatcacgg
     9661 cgtccccgga gaatgactgg ctgtggatct tctcagactt cagaagtgtg aaaatgacgt
     9721 acttgaccct cattacttac cagtctcacc tcctactcca gagggttgag agaaacttgt
     9781 ctaagagtat gagagctact ctgcgacaaa tgggttcctt aatgaggcaa gtgctgggtg
     9841 ggcacggaga agataccttg gagtcagacg atgacattca acgattacta aaagactctt
     9901 tgcgaaagac aaggtgggtg gatcaagagg tgcgccatgc agctagaacc atgaatggag
     9961 attacagccc cgacaagaaa gtatcccgta aggtaggatg ttcagaatgg gtctgctctg
    10021 ctcaacagat tgccgtctcc acctcagcca acccggcccc tgtctcagag cttgacatta
    10081 gggccctctc taagcggttt caaaacccct tgatctcggg cctgagagtg gttcagtggg
    10141 caaccggtgc ccattataag cttaagccta ttctagatga tctaaatgtt ttcccatctc
    10201 tctgtcttgt aattggagac gggtcagggg gaatatcaag ggcagttctc aacatgtttc
    10261 cagattctaa gcttgtgttc aacagcctat tggaggtgaa tgatctgatg gcttccggaa
    10321 cacatccact gcctccttca gcaatcatga gtggaggaga tgacatcatc tccagagtga
    10381 tagactttga ctcaatatgg gagaaaccgt ccgacctgag gaactcggcc acctggagat
    10441 acttccagtc ggttcaaaaa caggtcaaca tgtcgtatga cctcattatt tgtgatgcag
    10501 aagttactga tattgcatct atcaaccgga taactctgtt gatgtctgat ttcgcattgt
    10561 ctatagatgg accactttat ctggtcttca aaacttacgg gactatgcta gtaaacccgg
    10621 actataaagc tattcaacat ctgtcaagag cgttcccttc ggtcacaggg tttgtaaccc
    10681 aagtaacttc atccttttct tctgagctat acctccggtt ctctaaacga ggaaagtttt
    10741 tcagggacgc cgagtacttg acctcttcca cccttcgaga gatgagcctt gtgttgttca
    10801 attgcagcag ccccaaaagt gagatgcaga gagctcgttc cttaaactat caagacctgg
    10861 taaggggatt tcctgaagag atcatatcaa atccttacaa cgaaatgatc ataactctga
    10921 ttgacaatga tgtagagtcc ttcctagtcc acaagatggt ggatgatctt gagctacaga
    10981 ggggaactct gtctaaagtg gctatcatta tatccatcat gatcgttttt tccaatagag
    11041 tcttcaacat ttccaaacct ttgactgacc ccttgttcta tccaccgtct gatcctaaaa
    11101 tcctgaggca cttcaacata tgttgcagta ctatgatgta tctatctacc gctttaggcg
    11161 acgtccctaa cttcgcaaga cttcatgacc tgtataacag acctataact tgttacttca
    11221 gaaaacaagt tattcgaggg aatatttatc tatcttggag ttggtccgat gataccccag
    11281 tgttcaagag agtagcctgt aattctagct tgagtctgtc atctcactgg atcaggttga
    11341 tctacaagat agtgaagact accagactca ttggcagcat aaaagaccta tcaggagagg
    11401 tagaacgaca tcttcatggg tataacagat ggatcaccct cgaggatatc cgatctagat
    11461 catccctact agattacagt tgtttgtaag ccggatatta ccgaaagcct gtgcatgcta
    11521 aaattcttgt atgatgcatc ttgaaaaaaa caagatcttg aatccggacc tctggttgtt
    11581 tgattgtttt ttccatcttt attgtttttt tgttaagcgt gggtcggcat ggcatctcca
    11641 cctcctcgcg gtccgacctg ggcatccgaa ggaggacgca cgtccactcg gatggctaag
    11701 ggaggggcgg ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
    11761 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
    11821 tgctgaaagt cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    11881 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    11941 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    12001 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    12061 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    12121 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    12181 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    12241 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    12301 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    12361 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    12421 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    12481 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    12541 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    12601 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    12661 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
    12721 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    12781 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    12841 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    12901 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12961 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    13021 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    13081 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    13141 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    13201 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    13261 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    13321 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    13381 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    13441 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    13501 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    13561 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    13621 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    13681 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    13741 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    13801 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    13861 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    13921 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    13981 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    14041 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    14101 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    14161 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    14221 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    14281 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    14341 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    14401 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
    14461 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    14521 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    14581 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctagtta ttaatagtaa
    14641 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
    14701 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
    14761 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
    14821 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
    14881 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
    14941 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
    15001 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
    15061 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
    15121 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
    15181 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
    15241 acgactcact atagggagac ccaagctggc tagattaagc gtctgatgag tccgtgagga
    15301 cgaaacccgg cgtaccgggt c
//
