LOCUS       sequence-135712-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-ONL
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(912..929)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    918..1029
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 atgaacgtgc tggactcctt catcaactac tatgattccg agaagcacgc cgagaacgcc
       61 gtgatttttc tgcatggtaa cgctacctcc agctacctgt ggaggcacgt cgtgcctcac
      121 atcgagcccg tggctagatg catcatccct gatctgatcg gaatgggtaa gtccggcaag
      181 agcgggaatg gctcatatcg cctcctggat cactacaagt acctcaccgc ttggttcgag
      241 ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg actgggggag cgctctggcc
      301 tttcactacg cctacgagca ccaagacagg atcaaggcca tcgtccatat ggagagtgtc
      361 gtggacgtga tcgagtcctg gatggggtgg cctgacatcg aggaggagct ggccctgatc
      421 aagagcgaag agggcgagaa aatggtgctt gagaataact tcttcgtcga gaccctgttg
      481 ccaagcaaga tcatgcggaa actggagcct gaggagttcg ctgcctacct ggagccattc
      541 aaggagaagg gcgaggttag acggcctacc ctctcctggc ctcgcgagat ccctctcgtt
      601 aagggaggca agcccgacgt cgtccagatt gtccgcaact acaacgccta ccttcgggcc
      661 agcgacgatc tgcctaagct gttcatcgag tccgaccctg ggttcttttc caacgctatt
      721 gtcgagggag ctaagaagtt ccctaacacc gagttcgtga aggtgaaggg cctccacttc
      781 ctccaggagg acgctccaga tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg
      841 ctgaagaacg agcagtaatc tagagcggcc gctcgataag cttgatcgat gatctagagc
      901 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      961 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     1021 aattgcatcg cattgtctga gtaggtgtca ttctattctg ggg
//
