LOCUS       sequence-135714-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-ONL
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..55
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     polyA_signal    102..326
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     315..332
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag
       61 actgccttgg gaaaagctgc aggttctttc cgcctcagaa gccatagagc ccaccgcatc
      121 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
      181 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
      241 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
      301 agatggctgg caactagaag gcacagtcga ggctgatcag cgagctctag atcatcgatc
      361 aagcttatcg agcggccgct ctagattact gctcgttctt cagcacgcgc tccacgaagc
      421 tcttgatgta cttacccatt tcatctggag cgtcctcctg gaggaagtgg aggcccttca
      481 ccttcacgaa ctcggtgtta gggaacttct tagctccctc gacaatagcg ttggaaaaga
      541 acccagggtc ggactcgatg aacagcttag gcagatcgtc gctggcccga aggtaggcgt
      601 tgtagttgcg gacaatctgg acgacgtcgg gcttgcctcc cttaacgaga gggatctcgc
      661 gaggccagga gagggtaggc cgtctaacct cgcccttctc cttgaatggc tccaggtagg
      721 cagcgaactc ctcaggctcc agtttccgca tgatcttgct tggcaacagg gtctcgacga
      781 agaagttatt ctcaagcacc attttctcgc cctcttcgct cttgatcagg gccagctcct
      841 cctcgatgtc aggccacccc atccaggact cgatcacgtc cacgacactc tccatatgga
      901 cgatggcctt gatcctgtct tggtgctcgt aggcgtagtg aaaggccaga gcgctccccc
      961 agtcgtggcc cacaaagatg attttctttg gaaggttcag cagctcgaac caagcggtga
     1021 ggtacttgta g
//
