LOCUS       sequence-135719-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-YNL-CP
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             946..993
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
ORIGIN
        1 gtgaccgccg ccgggggtac caaggtgtac gaccccgagc agaggaagag gatgatcacc
       61 ggcccccagt ggtgggccag gtgcaagcag atgaacgtgc tggacagctt catcaactac
      121 tacgacagcg agaagcacgc cgagaacgcc gtgatcttcc tgcacggcaa cgccactagc
      181 agctacctgt ggaggcacgt ggtgccccac atcgagcccg tggccaggtg catcatcccc
      241 gatctgatcg gcatgggcaa gagcggcaag agcggcaacg gcagctacag gctgctggac
      301 cactacaagt acctgaccgc ctggttcgag cttctgaacc tgcccaagaa gatcatcttc
      361 gtgggccacg actggggcgc cgccctggcc ttccactacg cctacgagca ccaggacagg
      421 atcaaggcca tcgtgcacat ggagagcgtg gtggacgtga tcgagagctg ggacgagtgg
      481 ccagacatcg aggaggacat cgccctgatc aagagcgagg agggcgagaa gatggtgctg
      541 gagaacaact tcttcgtgga gaccgtgctg cccagcaaga tcatgagaaa gctggagccc
      601 gaggagttcg ccgcctacct ggagcccttc aaggagaagg gcgaggtgag aagacccacc
      661 ctgagctggc ccagagagat ccccctggtg aagggcggca agcccgacgt ggtgcagatc
      721 gtgagaaact acaacgccta cctgagagcc agcgacgacc tgcccaagct gttcatcgag
      781 ggcgaccccg gcttcttcag caacgccatc gtggagggcg ccaagaagtt ccccaacacc
      841 gagttcgtga aggtgaaggg cctgcacttc ctccaggagg acgcccccga cgagatgggc
      901 aagtacatca agagcttcgt ggagagagtg ctgaagaacg agcaggcttg caagaactgg
      961 ttcagtagct taagccactt tgtgatccac cttaacagcc acggcttccc tcccgaggtg
     1021 gaggagca
//
