LOCUS       sequence-135721-        8569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  promoterless Tol2 vector with mCherry tag upstream of and in frame 
            with attR1/attR2 cassette and late SV40 polyadenylation signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 366 pTol2-CherryDest
FEATURES             Location/Qualifiers
     source          1..8569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      1354..1363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1360..2067
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1505..1523)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1773..1792
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1967..1990
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    2080..2204
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2229..2234,2235..2252,2253..2259)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2313..2972
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2379..2398)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3314..3619
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3498..3517
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3660..3784)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    3824..3958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3848..3867)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3902..3921
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6099..6286,6287..6298)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6332..6348)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6332..6348)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6385..6405)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6385..6403)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6424..6440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6424..6440)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6437..6459)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6448..6464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6472..6478,6479..6496,6497..6502))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6517..6538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6655..6672)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6826..7414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6906..6925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7585..8376,8377..8445))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8208..8227
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8446..8550)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcgctagc atcgattcga attcaaggct agcgccacca tggtgagcaa gggcgaggag
     1381 gacaacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     1441 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1501 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     1561 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     1621 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     1681 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     1741 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     1801 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     1861 cagaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac cacctacaag
     1921 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagct ggacatcacc
     1981 tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg ccgccactcc
     2041 accggcggca tggacgagct gtacaagaga tctcgaatca caagtttgta caaaaaagct
     2101 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     2161 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
     2221 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
     2281 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
     2341 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     2401 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     2461 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     2521 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     2581 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     2641 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     2701 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     2761 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     2821 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     2881 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     2941 agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca
     3001 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     3061 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
     3121 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     3181 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     3241 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     3301 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     3361 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     3421 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     3481 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     3541 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     3601 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     3661 atagtgactg gatatgttgt gttttacagc attatgtagt ctgtttttta tgcaaaatct
     3721 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     3781 tggtgattcg agcctctaga actatagtga gtcgtattac gtagatccag acatgataag
     3841 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     3901 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3961 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     4021 attcgcggcc gcggcgccaa tgcattgggc cctcgagcat atgagatctg tcccgatctt
     4081 tctcttctgt gctgtcaatt tagagtagtt tttgaggtaa tttgggtgca ttctctgtaa
     4141 aaacaacaaa taaatattta tattgtatgc aataaatgta acaattacac atacactctc
     4201 tgattacagc attaaagggt tagttcaccc aaaaatgaaa agatgtcatt aatgactcac
     4261 cctcatgtcg ttccaagccc gtaacacctc cgttcatctt cggaacacag tttaagatat
     4321 tttagattta gtccgagagc tttctgtccc tccactgaaa atgtatgtac ggtatactgt
     4381 ccatgtccag aaaggtaata aaaacatcaa agtagtccat gtgacatcag tgggttaggt
     4441 agaattattt gaagcatcga aaatacattt tggtccaaaa ataacaaaac ctacgacttt
     4501 attcagcatt gtagcgtcac tgcgaagtcg tgaacgcgga ttgacaacag acccggaaga
     4561 gaatacaatg ccgaataaag tcgtaggttt tgttattttt ggaccaaaat gtattcgatg
     4621 cttcaaaaaa ttctaactaa cccactgatg tcacatggac tactttgatg tttttattac
     4681 ctttctggac atggacagta taccgtacat acattctcaa tggaggcaca gaaagctctc
     4741 ggactaaatc taaaatatct taaactgtgt tctgaagatg aacggaggtc ttacgggctt
     4801 ggaacgacat gagggcgagt cattaatgac attattttca tttttgggtg aactaaccct
     4861 ttaataaaac tgacatttgc agccaattgc acactttata atgttaaata cttgttgatt
     4921 ttagaaatac agtagcattt agtgacaatc aaacattaat tttatttctg attatattga
     4981 caaaaacaca ggactgtaag gctgacatga cagggtgaat tgatttcatt acattagttt
     5041 tgatggtaac acttttacaa taaggttcgt tagttaacta cattagttaa catgaactaa
     5101 taatgaactg cacgtataca tgttaatttc aacatttact aatactttat atcttgttaa
     5161 cattagttaa tgcactgtga actaacatga acaaacaatg aacatctgta tttctattaa
     5221 ctaacgttaa caaagattaa cagatacagt aacaaatgta ttgctcatgg ttatttaatg
     5281 ttagttaata catgaactaa tcaacctcat tgtaaagtgt taccgttttg acaatacaac
     5341 atgtttggaa aaaaatttct cgcttggttt tcttgtgttt tgcatgaatt caaacacaat
     5401 gagagcacgg aatgataatg attattcaaa gctgcaatag gcgccaaatt tcccgtggac
     5461 ctaacgttac caattacaaa tgcattttag cattttatta cgtgagcctt atattgtcaa
     5521 tcgcatgcat tagtgaaaat aggcctacta cacatataaa aggcgcgcca tcaacgtcaa
     5581 aacatgcaca cccaaaaaaa aaaaaaaaaa aaaaaaaaaa aactatcagt aaaacaaaat
     5641 acttaatgta cttacctcaa tatgcttcct taggtttgat ggcgaacttt tgaaggccga
     5701 tatttcttta ttaagcggga gacagaggac acatttcatc ttgaatgaat ctttatttac
     5761 accacttaaa gaaaagaatt cgcgaagata cggccacggg tgctcttgat cctgtggctg
     5821 attttggact gtgctgctcg cagctgctga tgaatcacat acttcctcca ttttcttcca
     5881 ctgattgact gttataattt ccctaatttc caggtcaagg tgctgtgcat tgtggtaata
     5941 gatgtgacat gacgtcactt ccaaaggacc aatgaacatg tctgaccaat ttcatataat
     6001 gtgaaaacga ttttcatagg cagaataaat aacatttaaa ttaaactggg catcagcgca
     6061 attcaattgg tttggtaata gcaagggaaa atagaatgaa gtgatctcca aaaaataagt
     6121 actttttgac tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg
     6181 taattaagta aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc
     6241 aaaaataata cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggt
     6301 tcttgacccc ctaccttcag caagcccagc agatccacta gttctagagc ggccgccacc
     6361 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     6421 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6481 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6541 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6601 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6661 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6721 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6781 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6841 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6901 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6961 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7021 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7081 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7141 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7201 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7261 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7321 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7381 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7441 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7501 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7561 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7621 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7681 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7741 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7801 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7861 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7921 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7981 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8041 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8101 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8161 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8221 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8281 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8341 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8401 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8461 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8521 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtg
//
