LOCUS       sequence-135722-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-YNL-CP
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..53
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     polyA_signal    100..324
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     313..330
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(387..506)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(510..557)
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
ORIGIN
        1 cagaggccac ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca tgctccagac
       61 tgccttggga aaagctgcag gttctttccg cctcagaagc catagagccc accgcatccc
      121 cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc
      181 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
      241 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
      301 atggctggca actagaaggc acagtcgagg ctgatcagcg agctctagat catcgatcaa
      361 gcttatcgag cggccgctct agattagacg ttgatcctgg cgctggcgca agcagcaggg
      421 tgtctatcca tgccgctctc ctgggcgcag ctcatgggca gggtgccggc ggcctgctcc
      481 tccacctcgg gagggaagcc gtggctgtta aggtggatca caaagtggct taagctactg
      541 aaccagttct tgcaagcctg ctcgttcttc agcactctct ccacgaagct cttgatgtac
      601 ttgcccatct cgtcgggggc gtcctcctgg aggaagtgca ggcccttcac cttcacgaac
      661 tcggtgttgg ggaacttctt ggcgccctcc acgatggcgt tgctgaagaa gccggggtcg
      721 ccctcgatga acagcttggg caggtcgtcg ctggctctca ggtaggcgtt gtagtttctc
      781 acgatctgca ccacgtcggg cttgccgccc ttcaccaggg ggatctctct gggccagctc
      841 agggtgggtc ttctcacctc gcccttctcc ttgaagggct ccaggtaggc ggcgaactcc
      901 tcgggctcca gctttctcat gatcttgctg ggcagcacgg tctcca
//
