LOCUS       sequence-135725-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, 
            Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole 
            ribonucleotide synthetase (EcPurK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Kappock
  TITLE     Single Trp variants of Acetobacter aceti PurE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK500
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgtcttcc cgcaataatc cggcgcgtgt
       61 cgccattgtg atggggtcca aaagcgactt tgctaccatg cagttcgccg ccgaaatctt
      121 cgaaatcctg aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa
      181 actgttcagc ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc
      241 aggcggcgca gcgcatctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg
      301 cgtgccagta cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat
      361 gccgcgcggc attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc
      421 gttactggca gcacaaattc ttgcgactca tgataaagaa ctgcaccagc gtctgaatga
      481 ctggcgcaaa gcccagaccg acgaagtgct ggaaaacccg gacccgcgag gtgcggcatg
      541 aaacaggttt gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa
      601 ccgttaggca ttgctgtctg gccagtcggg ctggacgctg aaccggcggc ggtgcctttt
      661 caacaaagcg tgattaccgc tgagatagaa cgctggccgg aaaccgcatt aacccgcgag
      721 ctggcgcgcc atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg
      781 actcagaagc agcttttcga taagctccac ctgccgactg caccgtggca gttacttgcc
      841 gaacgcagcg agtggcctgc ggtgtttgat cgtttaggtg agctggcgat tgttaagcgt
      901 cgcactggtg gttatgacgg tcgcggtcaa tggcgtttac gcgcaaatga aaccgaacag
      961 ttaccggcag agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa
     1021 gtgtc
//
