LOCUS       sequence-135731-       10622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 vector with fli1ep element upstream of mCherry, attR1/R2 
            cassette, and late SV40 pA signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 373 pTol2-fli1ep:CherryDest
FEATURES             Location/Qualifiers
     source          1..10622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      3407..3416
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3413..4120
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3558..3576)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3826..3845
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4020..4043
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    4133..4257
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4282..4287,4288..4305,4306..4312)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4366..5025
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4432..4451)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5367..5672
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5551..5570
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5713..5837)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    5877..6011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5901..5920)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5955..5974
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8152..8339,8340..8351)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8385..8401)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8385..8401)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8438..8458)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8438..8456)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8477..8493)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8477..8493)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8490..8512)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8501..8517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8525..8531,8532..8549,8550..8555))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8570..8591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8708..8725)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8879..9467)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8959..8978)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9638..10429,10430..10498))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10261..10280
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10499..10603)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 taattcgccc ttggagatct catctttgac ccataaacat acactaaaac ccaacagtca
     1381 actttatcaa atgaaatgag tgtaattaac tcaaaattta ctgaatgttg attctactca
     1441 ttcgaaaaga gttttaaact cagtgttgaa ggtagtgagt tcattaaata cctcattact
     1501 tcaacttaac tggagtaagt tcatggtact cacatagatt agtttagctc aaatggtttg
     1561 tagcagtcgg ttccctcaag cagtttgagt tgccttaact ttttgggttt tacagtactc
     1621 agttggtttg agttcacttc acttattggg ttcaacatgt gctcaaattg catcgtttaa
     1681 tcaaatagat taagttcaca gtactcatta ggattagttt ttgaacttaa atggtttgtt
     1741 gcagtcggtt tcctcaaacg gtttgagttt ccataacttt atgggttttt cagtgtagta
     1801 aacatatgca tccacagatt ataatagtaa cctgcatgtt ttttccttca caatttgaca
     1861 cagatacctc acatgattga cttttaactg tacactttag tcgacaatga gaagggtgtc
     1921 tcaatctatg ggtatttcct gttccacaca atctggtttc ctgtcaggtt tccttttcct
     1981 cctgaaacaa aagacaggaa tgccctctgt ataaaatagc acagcgcttt taatgggtct
     2041 ctgtacatcc cgacctcatg acgcatcttc tgcgggacag ctcaatcacg atcaagttgg
     2101 cttatgaaaa cagttttttg tccccctgtt aagttaaaag cacggtagca tacatgcatt
     2161 attcatcatg cagcatattc agcagtacaa aagatgagct cttgacacac ctcagcgtaa
     2221 aatctctatt ccagcgtaat cctgcggttc tggatggtta agcgagcctt gcaaacacgg
     2281 ccaatttata gccatgttga aaatagcagc tttgtgagca tttcgacctc aatgtaccaa
     2341 gaatgcgtgc tcggtaactg aaaaagaggg ggtggagatt ttggatttag agatggaatc
     2401 acatgcacgc tgtatatgct atataaaaat agcaggcatt taatgcatgc aaaatatgtt
     2461 tcgacactaa acactacaag tcggaaatat atctattttt ctttttttaa cgaaatgaga
     2521 catttaatgc gcagtaaatg tagtctaaca cgcacaatat tcctattatt gtccatgaaa
     2581 aatgtgaggt catatgctac tgatttaaag aaaatgttga acaggcacag cacacaagag
     2641 gtatcagcct ctctatatgg tccatacagc accaggctcg ctgcgttcat gtgcattcag
     2701 aggcgcgcac acgtgtggag cagacagact cgacgcagcg ggctttgctt tttattcatg
     2761 gaagcccatt ttagaagaag ctcgggcaga ctcgtcacat tcttcacttc accataggta
     2821 gatcaaatca aagttattgg atataacgga acaatattca tcatattatg agattattat
     2881 taagaccgag tgcattagac atattttgta tttcgatcgt tggaaaaagt atatatttta
     2941 ttttagcctt aaactatggt tagcctatat taatcagcat aatctgttat ttcagacgtt
     3001 gtattaatga ttatttctag cacagctttg atgcatcaat gcaatgtttc tacaaacccg
     3061 ccttgtttaa tgtcggaaaa aaagcgcaac aaatgtaaag agttggcagg cattctgtaa
     3121 atatttccag gcaaaaacat gcacaaagga aagaaacacc attgttttgg tgtcttatct
     3181 tcgctggaca aatatcctgc tttgggtgcc gtaatgaagg cagggccagt gacacacaca
     3241 catacacaca cacacataca cgcatacacg gcttccttct caagcgcagt gacttcactt
     3301 cccaaattta gaggaaaaaa aaccatccgg cgaaactgtc tctgtctctc cgccacatat
     3361 cgggggctgg aattaattca gacgcgaagg gcgaattatc gctagcgcca ccatggtgag
     3421 caagggcgag gaggacaaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     3481 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     3541 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     3601 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     3661 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     3721 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     3781 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     3841 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     3901 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa
     3961 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     4021 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga
     4081 gggccgccac tccaccggcg gcatggacga gctgtacaag agatctcgaa tcacaagttt
     4141 gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
     4201 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggcg
     4261 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
     4321 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     4381 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     4441 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     4501 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     4561 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     4621 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     4681 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     4741 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     4801 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     4861 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     4921 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     4981 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc
     5041 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     5101 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     5161 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     5221 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     5281 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     5341 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     5401 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     5461 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     5521 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     5581 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     5641 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     5701 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     5761 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     5821 ttcttgtaca aagtggtgat tcgagcctct agaactatag tgagtcgtat tacgtagatc
     5881 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     5941 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     6001 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     6061 gggaggtttt ttaattcgcg gccgcggcgc caatgcattg ggccctcgag catatgagat
     6121 ctgtcccgat ctttctcttc tgtgctgtca atttagagta gtttttgagg taatttgggt
     6181 gcattctctg taaaaacaac aaataaatat ttatattgta tgcaataaat gtaacaatta
     6241 cacatacact ctctgattac agcattaaag ggttagttca cccaaaaatg aaaagatgtc
     6301 attaatgact caccctcatg tcgttccaag cccgtaacac ctccgttcat cttcggaaca
     6361 cagtttaaga tattttagat ttagtccgag agctttctgt ccctccactg aaaatgtatg
     6421 tacggtatac tgtccatgtc cagaaaggta ataaaaacat caaagtagtc catgtgacat
     6481 cagtgggtta ggtagaatta tttgaagcat cgaaaataca ttttggtcca aaaataacaa
     6541 aacctacgac tttattcagc attgtagcgt cactgcgaag tcgtgaacgc ggattgacaa
     6601 cagacccgga agagaataca atgccgaata aagtcgtagg ttttgttatt tttggaccaa
     6661 aatgtattcg atgcttcaaa aaattctaac taacccactg atgtcacatg gactactttg
     6721 atgtttttat tacctttctg gacatggaca gtataccgta catacattct caatggaggc
     6781 acagaaagct ctcggactaa atctaaaata tcttaaactg tgttctgaag atgaacggag
     6841 gtcttacggg cttggaacga catgagggcg agtcattaat gacattattt tcatttttgg
     6901 gtgaactaac cctttaataa aactgacatt tgcagccaat tgcacacttt ataatgttaa
     6961 atacttgttg attttagaaa tacagtagca tttagtgaca atcaaacatt aattttattt
     7021 ctgattatat tgacaaaaac acaggactgt aaggctgaca tgacagggtg aattgatttc
     7081 attacattag ttttgatggt aacactttta caataaggtt cgttagttaa ctacattagt
     7141 taacatgaac taataatgaa ctgcacgtat acatgttaat ttcaacattt actaatactt
     7201 tatatcttgt taacattagt taatgcactg tgaactaaca tgaacaaaca atgaacatct
     7261 gtatttctat taactaacgt taacaaagat taacagatac agtaacaaat gtattgctca
     7321 tggttattta atgttagtta atacatgaac taatcaacct cattgtaaag tgttaccgtt
     7381 ttgacaatac aacatgtttg gaaaaaaatt tctcgcttgg ttttcttgtg ttttgcatga
     7441 attcaaacac aatgagagca cggaatgata atgattattc aaagctgcaa taggcgccaa
     7501 atttcccgtg gacctaacgt taccaattac aaatgcattt tagcatttta ttacgtgagc
     7561 cttatattgt caatcgcatg cattagtgaa aataggccta ctacacatat aaaaggcgcg
     7621 ccatcaacgt caaaacatgc acacccaaaa aaaaaaaaaa aaaaaaaaaa aaaaactatc
     7681 agtaaaacaa aatacttaat gtacttacct caatatgctt ccttaggttt gatggcgaac
     7741 ttttgaaggc cgatatttct ttattaagcg ggagacagag gacacatttc atcttgaatg
     7801 aatctttatt tacaccactt aaagaaaaga attcgcgaag atacggccac gggtgctctt
     7861 gatcctgtgg ctgattttgg actgtgctgc tcgcagctgc tgatgaatca catacttcct
     7921 ccattttctt ccactgattg actgttataa tttccctaat ttccaggtca aggtgctgtg
     7981 cattgtggta atagatgtga catgacgtca cttccaaagg accaatgaac atgtctgacc
     8041 aatttcatat aatgtgaaaa cgattttcat aggcagaata aataacattt aaattaaact
     8101 gggcatcagc gcaattcaat tggtttggta atagcaaggg aaaatagaat gaagtgatct
     8161 ccaaaaaata agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt
     8221 ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat ttttaattgt actcaagtaa
     8281 agtaaaaatc cccaaaaata atacttaagt acagtaatca agtaaaatta ctcaagtact
     8341 ttacacctct ggttcttgac cccctacctt cagcaagccc agcagatcca ctagttctag
     8401 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     8461 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8521 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8581 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8641 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8701 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8761 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8821 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8881 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8941 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9001 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9061 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9121 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9181 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9241 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9301 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9361 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9421 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9481 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9541 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9601 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9661 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9721 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9781 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9841 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9901 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9961 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10021 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10081 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10141 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10201 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10261 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10321 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10381 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10441 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10501 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10561 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10621 tg
//
