LOCUS       sequence-135736-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-CNL-CP
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             114..134
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             138..158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             162..182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(195..197,198..200,201..713)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(240..261)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(501..520)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     848..869
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctcgtttag tgaaccgtca gatcgcatcg attcgaattc cagcttggca ttccggtact
       61 gttggtaaaa agcttggcat tccggtactg ttggtaaagc caccatgctg gtaccaaaaa
      121 agaagagaaa ggttgtacca aaaaagaaga gaaaggttgt accaaaaaag aagagaaagg
      181 ttttcgaagg gcccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      241 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      301 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      361 tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc
      421 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      481 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      541 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      601 acatcctggg gcacaagctg gagtacaact actttagcga caacgtctat atcaccgccg
      661 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
      721 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      781 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
      841 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gggtaccaag gtgtacgacc
      901 ccgagcagag gaagaggatg atcaccggcc cccagtggtg ggccaggtgc aagcagatga
      961 acgtgctgga cagcttcatc aactactacg ac
//
