LOCUS       sequence-135741-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-CNL-CP
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..47
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     polyA_signal    94..318
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     307..324
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(381..500)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(504..551)
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
ORIGIN
        1 ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt
       61 gggaaaagct gcaggttctt tccgcctcag aagccataga gcccaccgca tccccagcat
      121 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
      181 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
      241 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
      301 ggcaactaga aggcacagtc gaggctgatc agcgagctct agatcatcga tcaagcttat
      361 cgagcggccg ctctagatta gacgttgatc ctggcgctgg cgcaagcagc agggtgtcta
      421 tccatgccgc tctcctgggc gcagctcatg ggcagggtgc cggcggcctg ctcctccacc
      481 tcgggaggga agccgtggct gttaaggtgg atcacaaagt ggcttaagct actgaaccag
      541 ttcttgcaag cctgctcgtt cttcagcact ctctccacga agctcttgat gtacttgccc
      601 atctcgtcgg gggcgtcctc ctggaggaag tgcaggccct tcaccttcac gaactcggtg
      661 ttggggaact tcttggcgcc ctccacgatg gcgttgctga agaagccggg gtcgccctcg
      721 atgaacagct tgggcaggtc gtcgctggct ctcaggtagg cgttgtagtt tctcacgatc
      781 tgcaccacgt cgggcttgcc gcccttcacc agggggatct ctctgggcca gctcagggtg
      841 ggtcttctca cctcgccctt ctccttgaag ggctccaggt aggcggcgaa ctcctcgggc
      901 tccagctttc tcatgatctt gctgggcagc acggtctcca cgaagaagtt gttctccagc
      961 accatcttct cgccctcctc gctcttgatc agggcgatgt cctcctcgat gtctggccac
     1021 tcgtcccagc tctcgatcac gtccaccacg ctctccatgt gcacga
//
