LOCUS       sequence-135745-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-ONL-CP
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             114..134
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             138..158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             162..182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             195..848
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             195..848
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gctcgtttag tgaaccgtca gatcgcatcg attcgaattc cagcttggca ttccggtact
       61 gttggtaaaa agcttggcat tccggtactg ttggtaaagc caccatgctg gtaccaaaaa
      121 agaagagaaa ggttgtacca aaaaagaaga gaaaggttgt accaaaaaag aagagaaagg
      181 ttttcgaagg gcccatggtg agcgtgatca agcccgagat gaagatgagg tactacatgg
      241 acggctccgt caatgggcat gagttcacaa tcgagggtga gggcacaggc agaccttacg
      301 agggacatca ggagatgaca ctgcgcgtca caatggccga gggcgggcca atgcctttcg
      361 ccttcgacct ggtgtcccac gtgttctgtt acggccacag agtttttact aaatatccag
      421 aagagatccc agactatttc aagcaggcct ttcctgaggg cctgtcctgg gagaggtccc
      481 tggagttcga ggacggcggc tccgcctccg tgagcgccca catcagcctg aggggcaaca
      541 ccttctacca caagtccaag ttcaccggcg tgaacttccc cgccgacggc cccatcatgc
      601 agaaccagag cgtggactgg gagccctcca ccgagaagat caccgccagc gacggcgtgc
      661 tgaagggcga cgtgaccatg tacctgaagc tggagggcgg cggcaaccac aagtgccaga
      721 tgaagaccac ctacaaggcc gccaaggaga tcctggagat gcccggcgac cactacatcg
      781 gccacaggct ggtgaggaag accgagggca acatcaccga gcaggtggag gacgccgtgg
      841 cccactccgg taccaaggtg tacgaccccg agcaacgcaa acgcatgatc actgggcctc
//
