LOCUS       sequence-135747-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-ONL-CP
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..55
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     polyA_signal    102..326
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     315..332
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(389..508)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(512..559)
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
ORIGIN
        1 gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag
       61 actgccttgg gaaaagctgc aggttctttc cgcctcagaa gccatagagc ccaccgcatc
      121 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
      181 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
      241 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
      301 agatggctgg caactagaag gcacagtcga ggctgatcag cgagctctag atcatcgatc
      361 aagcttatcg agcggccgct ctagattaga cgttgatcct ggcgctggcg caagcagcag
      421 ggtgtctatc catgccgctc tcctgggcgc agctcatggg cagggtgccg gcggcctgct
      481 cctccacctc gggagggaag ccgtggctgt taaggtggat cacaaagtgg cttaagctac
      541 tgaaccagtt cttgcaagcc tgctcgttct tcagcacgcg ctccacgaag ctcttgatgt
      601 acttacccat ttcatctgga gcgtcctcct ggaggaagtg gaggcccttc accttcacga
      661 actcggtgtt agggaacttc ttagctccct cgacaatagc gttggaaaag aacccagggt
      721 cggactcgat gaacagctta ggcagatcgt cgctggcccg aaggtaggcg ttgtagttgc
      781 ggacaatctg gacgacgtcg ggcttgcctc ccttaacgag agggatctcg cgaggccagg
      841 agagggtagg ccgtctaacc tcgcccttct ccttgaatgg ctccaggtag gcagcgaact
      901 cctcaggctc cagtttccgc atgatcttgc ttggcaacag ggtctcgacg aagaagttat
      961 tctcaagcac cattttctcg ccctcttcgc tcttgatcag ggccagctcc tcctcgatgt
     1021 caggccacc
//
