LOCUS       sequence-135749-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-ONL-CP
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             850..897
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             901..1020
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
ORIGIN
        1 gtgctggact ccttcatcaa ctactatgat tccgagaagc acgccgagaa cgccgtgatt
       61 tttctgcatg gtaacgctac ctccagctac ctgtggaggc acgtcgtgcc tcacatcgag
      121 cccgtggcta gatgcatcat ccctgatctg atcggaatgg gtaagtccgg caagagcggg
      181 aatggctcat atcgcctcct ggatcactac aagtacctca ccgcttggtt cgagctgctg
      241 aaccttccaa agaaaatcat ctttgtgggc cacgactggg ggagcgctct ggcctttcac
      301 tacgcctacg agcaccaaga caggatcaag gccatcgtcc atatggagag tgtcgtggac
      361 gtgatcgagt cctggatggg gtggcctgac atcgaggagg agctggccct gatcaagagc
      421 gaagagggcg agaaaatggt gcttgagaat aacttcttcg tcgagaccct gttgccaagc
      481 aagatcatgc ggaaactgga gcctgaggag ttcgctgcct acctggagcc attcaaggag
      541 aagggcgagg ttagacggcc taccctctcc tggcctcgcg agatccctct cgttaaggga
      601 ggcaagcccg acgtcgtcca gattgtccgc aactacaacg cctaccttcg ggccagcgac
      661 gatctgccta agctgttcat cgagtccgac cctgggttct tttccaacgc tattgtcgag
      721 ggagctaaga agttccctaa caccgagttc gtgaaggtga agggcctcca cttcctccag
      781 gaggacgctc cagatgaaat gggtaagtac atcaagagct tcgtggagcg cgtgctgaag
      841 aacgagcagg cttgcaagaa ctggttcagt agcttaagcc actttgtgat ccaccttaac
      901 agccacggct tccctcccga ggtggaggag caggccgccg gcaccctgcc catgagctgc
      961 gcccaggaga gcggcatgga tagacaccct gctgcttgcg ccagcgccag gatcaacgtc
     1021 taatctagag cggccgctcg ataagcttga tcgatgatct agagctcgct ga
//
