LOCUS       sequence-135751-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase
            with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Mullins EA, Kappock TJ
  TITLE     Crystal structures of Acetobacter aceti succinyl-coenzyme A 
            (CoA):acetate CoA-transferase reveal specificity determinants and 
            illustrate the mechanism used by class I CoA-transferases.
  JOURNAL   Biochemistry. 2012 Oct 23;51(42):8422-34. doi: 10.1021/bi300957f. 
            Epub 2012 Oct 11.
  PUBMED    23030530
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi300957f";
            journalName: "Biochemistry"; date: "2012-10-23- 23"; volume: "51"; 
            issue: "42"; pages: "8422-34"
COMMENT     Sequence Label: pJK517
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgatga tgggagcatg
       61 gtgcccgtat caataaaccg ctgatgccaa gacagagctt ccgtcagcag atgcggtgtg
      121 tgcttaccaa aggaattttt aagcgcacga tcaaaataat cctgcaacat cggccggtaa
      181 tccggatgcg cacatttgga aataatttca cgcgcacgtt gcaccggtga aagcccacgc
      241 agatccgcca gcccctgatc ggttacaaaa atctgcgcat cctgcatgat gtggtccaca
      301 tgcgcagcca tcggcacaat ggctgaaatc ttgccacctt tggctgtgga gggagacagg
      361 aagatggaca ggtaggaact ccgggcaaaa tcacccgatc ccccaatacc attcatcatt
      421 ttggaaccca tcacgcgggt agaattcacg tttccgtaaa tatccgcctc aatcatgccg
      481 ttcatggcaa tgcagcctaa acggcggata atgccggggc tgttgctgac atcctgctgg
      541 cgcagaatga tcttgctgcg gaagaaatcc atccggttat tgatttcttc cgcagcttcc
      601 gggctcagcg agaaagacga ggcagaggca atacgcatac ggccagaatc cagcatggcc
      661 aacatgccat cctgaatcac ctcactatag cccaccagat tttcaaatgg gccttccttc
      721 agcccttcca gcacggcgtt ggccacattg cccacaccag actggagcgg cagaagtgag
      781 ggcggcaaac ggttctgttt gacttcatgc ccaaagaaat caagcaaata gcccgcaata
      841 gcttttgctg tttcatccgg cgcggcaaac ggggcatttc tgtcccggtc attcgtgcgt
      901 acaatggcag caatcttgtc cgggttcacc cgtaaaaccg ggccacccac gcgctgatct
      961 gcccgtacaa tcggcacaat atcacgcgta ggcacgccac ttacgttgcc atcccagata
     1021 tcatggatgc cttccagcat cggattctgc
//
