LOCUS       sequence-135757-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase
            with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Mullins EA, Kappock TJ
  TITLE     Crystal structures of Acetobacter aceti succinyl-coenzyme A 
            (CoA):acetate CoA-transferase reveal specificity determinants and 
            illustrate the mechanism used by class I CoA-transferases.
  JOURNAL   Biochemistry. 2012 Oct 23;51(42):8422-34. doi: 10.1021/bi300957f. 
            Epub 2012 Oct 11.
  PUBMED    23030530
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi300957f";
            journalName: "Biochemistry"; date: "2012-10-23- 23"; volume: "51"; 
            issue: "42"; pages: "8422-34"
COMMENT     Sequence Label: pJK564
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgaca gagcgcattc ggaacgtcgc
       61 gctgcgcagt aaagtatgtc cggcagaaac cgcatccgag ttgatcaagc acggagacgt
      121 ggtggggacc agcggtttta ccggggcagg ttaccccaag gaagtaccca aggcgctggc
      181 ccagcggatg gaagctgcgc atgatcgggg cgaaaaatac cagatcagcc tgattacggg
      241 cgcagccaca ggccctcagc tggatggtga gctggccaag gccaacgggg tgtatttccg
      301 ctcgccattc aatacggatg ccaccatgcg caaccgtatt aatgcgggtg aaacggaata
      361 ttttgataac catctggggc aggtggctgg ccgtgccgtg cagggcaatt acggcaagtt
      421 caatattgcg ctggtggaag ccacagccat tacggaagat ggcggcattg tgcccacatc
      481 ttctgtcggc aattcccaga cattcctcaa tctggccgaa aaggtcatta tcgaggtaaa
      541 tgaatggcag aatccgatgc tggaaggcat ccatgatatc tgggatggca acgtaagtgg
      601 cgtgcctacg cgtgatattg tgccgattgt acgggcagat cagcgcgtgg gtggcccggt
      661 tttacgggtg aacccggaca agattgctgc cattgtacgc acgaatgacc gggacagaaa
      721 tgccccgttt gccgcgccgg atgaaacagc aaaagctatt gcgggctatt tgcttgattt
      781 ctttgggcat gaagtcaaac agaaccgttt gccgccctca cttctgccgc tccagtctgg
      841 tgtgggcaat gtggccaacg ccgtgctgga agggctgaag gaaggcccat ttgaaaatct
      901 ggtgggctat agtgaggtga ttcaggatgg catgttggcc atgctggatt ctggccgtat
      961 gcgtattgcc tctgcctcgt ctttctcgct gagcccggaa gctgcggaag aaatcaataa
     1021 ccggatggat ttcttccgca gcaagatcat tctgcgc
//
