LOCUS       sequence-135758-         638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-Golgi-7
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      272..281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             278..598
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
ORIGIN
        1 atccgctagc atgaggcttc gggagccgct cctgagcggc agcgccgcga tgccaggcgc
       61 gtccctacag cgggcctgcc gcctgctcgt ggccgtctgc gctctgcacc ttggcgtcac
      121 cctcgtttac tacctggctg gccgcgacct gagccgcctg ccccaactgg tcggagtctc
      181 cacaccgctg cagggcggct cgaacagtgc cgccgccatc gggcagtcct ccggggagct
      241 ccggaccgga ggggccaagg atccaccggt cgccaccatg gagaaaagtt tcgtgataac
      301 tgatccacgg ctgccagaca atcccatcat cttcgcatcc gatggcttcc tggagctgac
      361 cgagtattcc agagaggaga tcctgggccg caatggccgc tttctgcagg gaccagagac
      421 agaccaggcc acagtgcaga agattcgcga tgccattaga gatcagcgcg agattaccgt
      481 gcagctgata aactacacaa aaagcgggaa gaaattctgg aacctcctgc acctccagcc
      541 catgagggac cagaagggtg agctccagta tttcatcgga gtgcagctgg atggataagc
      601 ggccgcgact ctagatcata atcagccata ccacattt
//
