LOCUS       sequence-135761-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with 
            C-terminal His6 tag (YfdEH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Mullins EA, Sullivan KL, Kappock TJ
  TITLE     Function and X-ray crystal structure of Escherichia coli YfdE.
  JOURNAL   PLoS One. 2013 Jul 23;8(7):e67901. doi: 
            10.1371/journal.pone.0067901. Print 2013.
  PUBMED    23935849
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-23"; volume: "8(7):e67901. doi"; pages: " 
            10.1371/journal.pone.0067901. Print 2013"
COMMENT     Sequence Label: pJK594
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgatgatg
       61 agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc ggcataacat
      121 gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt cccccggctt
      181 caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc acacttaata
      241 acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca gctgcctgcg
      301 tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg cgtaaaatat
      361 tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg cataacgcag
      421 aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta ttgaaaacat
      481 caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc ccagttgcga
      541 tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc gctatatcga
      601 catgcgcccc tctctggctc ttttcgcgac cataaagtgc actcactatt ccgctgaata
      661 aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc ggagcatcag
      721 gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg taggcaggag
      781 catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg aggcgcgggt
      841 tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct gggcgaaaat
      901 tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta tcgtgatcat
      961 tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa tagagtgact
     1021 gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc ggctcaactt
     1081 taattacc
//
