LOCUS       sequence-135762-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic Ca Biosensor, Excitation: 433 / 515, 
            Emission: 475 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  TITLE     ECFP/Venus cytosolic calcium biosensor
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YC 3.6 Cameleon
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(36..57)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(297..316)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(573..591)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(574..591)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(611..628)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    617..728
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
       61 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      121 ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      181 ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
      241 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      301 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      361 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      421 acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
      481 ccaacttcaa gatccgccac aacatcgagt aagaattctg cagatatcca tcacactggc
      541 ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
      601 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      661 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      721 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
      781 agcaaggggg aggattggga agacaatagc aggcatgctg g
//
