LOCUS       sequence-135763-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase 
            (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Mullins EA, Sullivan KL, Kappock TJ
  TITLE     Function and X-ray crystal structure of Escherichia coli YfdE.
  JOURNAL   PLoS One. 2013 Jul 23;8(7):e67901. doi: 
            10.1371/journal.pone.0067901. Print 2013.
  PUBMED    23935849
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-23"; volume: "8(7):e67901. doi"; pages: " 
            10.1371/journal.pone.0067901. Print 2013"
COMMENT     Sequence Label: pJK591
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 taattttgtt taactttaag aaggagatat accatggctg aaaccacgcc agcaaaaccg
       61 aaggggccct ttgatgggct cctcgttatt gatctgacac atgtgctgaa tggtccgttc
      121 ggcacaacaa ttctaacaga tctgggggct cgtacaatca agatcgagcc accggggcat
      181 ggggatgata cgcgtaccta tggcccatat gttggagacc aatcgcttta cttctctttt
      241 gtaaacaggg gaaaggaaag cattgtcctt aacctcaagg atgagggcga tcgagcgatt
      301 tttctggaaa tggtgcgtaa ggccgatgtg ctggcggaaa acttccgccc cggcgtgatg
      361 gaccgtttgg gtttcaatta tgaggaactg gcaaagatca atccacggtt gatttatgca
      421 tcttcatcag ggttcggtca gacaggcccg ctcgcacatt atcctgccta cgatactatt
      481 gtgcaggcca tgagcggcat catgatggcc acgggctttc ccgatggtcc gccaacgcgg
      541 gtagggggca catcgctttc tgctctgtgc ggtggggttt tcatgttctg cggcattgcc
      601 agtgcgcttt atgcgcgtga gcgcacaggg aaaggggcac acattgatgt ttcaatgttt
      661 gatggtacct tggcattttt gcagcatgct ctgatgtgct ggtctgctac aggcaaagcc
      721 cctgcgcgta ttggcaatcg ccatccttat atggcgcctt ttgatgtgtt tcaggcgcag
      781 gacaagcctt ttgtcatctg ttgtgggaat gaccatcttt ttaaggcgct ttgcgatgtg
      841 attggtgcgc ctgaactggc gacagacccg cgttttgtcg aaaatcatga tagaatggcc
      901 aataatgatg cgctgaaagc tgcattggaa aaagcacttt ccaaacagcc agcggcgcat
      961 tggctggatg ttattcacaa agccggtgtg
//
