LOCUS       sequence-135764-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase 
            (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Mullins EA, Sullivan KL, Kappock TJ
  TITLE     Function and X-ray crystal structure of Escherichia coli YfdE.
  JOURNAL   PLoS One. 2013 Jul 23;8(7):e67901. doi: 
            10.1371/journal.pone.0067901. Print 2013.
  PUBMED    23935849
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-23"; volume: "8(7):e67901. doi"; pages: " 
            10.1371/journal.pone.0067901. Print 2013"
COMMENT     Sequence Label: pJK591
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcagg cgtcattttg cctctggtgc ggcaaattcc
      121 ttacgcaacg ccgcgccctg ctcatccagc ttaggggcgc cgggccgttc atgtgggtca
      181 tcatacccac tgattttcac cgggttaccg ggcatcatca ccccgccaga tttaatcagc
      241 atattccgcg cggctgtttg tggcagattt gccgcctctg ccacgtccag caacggccca
      301 acaggcacac cggctttgtg aataacatcc agccaatgcg ccgctggctg tttggaaagt
      361 gctttttcca atgcagcttt cagcgcatca ttattggcca ttctatcatg attttcgaca
      421 aaacgcgggt ctgtcgccag ttcaggcgca ccaatcacat cgcaaagcgc cttaaaaaga
      481 tggtcattcc cacaacagat gacaaaaggc ttgtcctgcg cctgaaacac atcaaaaggc
      541 gccatataag gatggcgatt gccaatacgc gcaggggctt tgcctgtagc agaccagcac
      601 atcagagcat gctgcaaaaa tgccaaggta ccatcaaaca ttgaaacatc aatgtgtgcc
      661 cctttccctg tgcgctcacg cgcataaagc gcactggcaa tgccgcagaa catgaaaacc
      721 ccaccgcaca gagcagaaag cgatgtgccc cctacccgcg ttggcggacc atcgggaaag
      781 cccgtggcca tcatgatgcc gctcatggcc tgcacaatag tatcgtaggc aggataatgt
      841 gcgagcgggc ctgtctgacc gaaccctgat gaagatgcat aaatcaaccg tggattgatc
      901 tttgccagtt cctcataatt gaaacccaaa cggtccatca cgccggggcg gaagttttcc
      961 gccagcacat cggccttacg caccatttcc agaaaaatcg ctcgatcgcc ctcatccttg
     1021 aggttaagga caatgctttc ctttcccctg tttacaaa
//
