LOCUS       sequence-135767-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation:448 / 473, Emission:500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: miniSOG-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..352
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     polyA_signal    591..712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(628..647)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     682..701
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggag aaaagtttcg tgataactga
       61 tccacggctg ccagacaatc ccatcatctt cgcatccgat ggcttcctgg agctgaccga
      121 gtattccaga gaggagatcc tgggccgcaa tggccgcttt ctgcagggac cagagacaga
      181 ccaggccaca gtgcagaaga ttcgcgatgc cattagagat cagcgcgaga ttaccgtgca
      241 gctgataaac tacacaaaaa gcgggaagaa attctggaac ctcctgcacc tccagcccat
      301 gagggaccag aagggtgagc tccagtattt catcggagtg cagctggatg gatccggact
      361 cagatctaag ctgaaccctc ctgatgagag tggccccggc tgcatgagct gcaagtgtgt
      421 gctctcctga gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat
      481 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
      541 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
      601 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
      661 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
      721 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tt
//
