LOCUS       sequence-135776-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-Cas9 induced gene disruption.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Kim D, Fiske BP, Birsoy K, Freinkman E, Kami K, Possemato RL, 
            Chudnovsky Y, Pacold ME, Chen WW, Cantor JR, Shelton LM, Gui DY, 
            Kwon M, Ramkissoon SH, Ligon KL, Kang SW, Snuderl M, Vander Heiden 
            MG, Sabatini DM
  TITLE     SHMT2 drives glioma cell survival in ischaemia but imposes a 
            dependence on glycine clearance.
  JOURNAL   Nature. 2015 Apr 16;520(7547):363-7. doi: 10.1038/nature14363. Epub 
            2015 Apr 8.
  PUBMED    25855294
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14363"; journalName: "Nature"; date: "2015-04-16- 
            16"; volume: "520"; issue: "7547"; pages: "363-7"
COMMENT     Sequence Label: sgGCAT_2
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(311..331)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(338..361)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      361..370
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(390..601)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    670..787
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agctgtcgtc caccttggcc atctcgttgc tgaagatctc ttgcagatag cagatccggt
       61 tcttccgtct ggtgtatctt cttctggcgg ttctcttcag ccgggtggcc tcggctgttt
      121 cgccgctgtc gaacagcagg gctccgatca ggttcttctt gatgctgtgc cggtcggtgt
      181 tgcccagcac cttgaatttc ttgctgggca ccttgtactc gtcggtgatc acggcccagc
      241 ccacagagtt ggtgccgatg tccaggccga tgctgtactt cttgtcggct gctgggactc
      301 cgtggatacc gaccttccgc ttcttctttg gggccatctt atcgtcatcg tctttgtaat
      361 ccatggtggc ggatcccgcg tcacgacacc tgtgttctgg cggcaaaccc gttgcgaaaa
      421 agaacgttca cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg
      481 cggagccagt acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg
      541 gttcaattgc cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc
      601 cactgacggg caccggagcc actcgagccg gcgccaaagt ggatctctgc tgtccctgta
      661 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
      721 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
      781 tttaaaattg tggatgaata ctgccatttg tctcaagatc tagaattcaa aaaagcaccg
      841 actcggtgcc actttttcaa gttgataacg
//
