LOCUS       sequence-135779-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 thioredoxin with Cys35>Ser mutant 
            (AaTrxA-C35S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK561
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             402..419
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(472..490)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      486..533
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      570..998
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(657..676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     867..888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgagtgaa catacgcttg ctgtcagcga
       61 ttccagcttt gatcaagatg ttctcaaggc ctctggcctt gtgctggtag atttctgggc
      121 agaatggtgc gggcccagca aaatgattgg cccggcattg ggagaaattg gcaaggaatt
      181 tgccggcaag gtaacggttg ccaaggtaaa tattgatgac aacccggaaa ctccaaatgc
      241 gtatcaggtg cgtagcattc caacccttat gctggtgcgt gatggcaagg ttatagataa
      301 aaaagtgggt gccctgccca aaagccagtt gaaggcatgg gtagaaagcg ctcagtaatt
      361 agaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
      421 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      481 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      541 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      601 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      661 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      781 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      841 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
      901 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
      961 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aac
//
