LOCUS       sequence-135780-        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 thioredoxin with Cys35>Ser mutant 
            (AaTrxA-C35S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK561
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             425..447
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(478..497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(479..497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattctaa ttactgagcg ctttctaccc atgccttcaa
      121 ctggcttttg ggcagggcac ccactttttt atctataacc ttgccatcac gcaccagcat
      181 aagggttgga atgctacgca cctgatacgc atttggagtt tccgggttgt catcaatatt
      241 taccttggca accgttacct tgccggcaaa ttccttgcca atttctccca atgccgggcc
      301 aatcattttg ctgggcccgc accattctgc ccagaaatct accagcacaa ggccagaggc
      361 cttgagaaca tcttgatcaa agctggaatc gctgacagca agcgtatgtt cactcatatg
      421 tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt gtggtctccc
      481 tatagtgagt cgtattaatt tcgcgggatc gagatctcgg gcagcgttgg gtcctggcca
      541 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
      601 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
      661 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
      721 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
      781 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
      841 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
      901 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
      961 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
     1021 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctgg
//
