LOCUS       sequence-135781-        5215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of mEos2-MTSBs in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Buss J, Coltharp C, Shtengel G, Yang X, Hess H, Xiao J
  TITLE     A multi-layered protein network stabilizes the Escherichia coli 
            FtsZ-ring and modulates constriction dynamics.
  JOURNAL   PLoS Genet. 2015 Apr 7;11(4):e1005128. doi: 
            10.1371/journal.pgen.1005128. eCollection 2015 Apr.
  PUBMED    25848771
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2015-04-7"; volume: "11(4):e1005128. doi"; pages: " 
            10.1371/journal.pgen.1005128. eCollection 2015 Apr"
COMMENT     Sequence Label: pXY029 (mEos2-MTSBs)
FEATURES             Location/Qualifiers
     source          1..5215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(80..94,95..100,101..117,118..124)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    132..148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             167..178
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             191..868
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     terminator      983..1077
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1147..1169)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        1633..1735
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1736..2395
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1802..1821)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2944..3532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3433..3452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3686..3703
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3776..3853
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3854..4936
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3873..3892)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(4985..4990,4991..5008,5009..5015)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5023..5039
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5047..5063
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5047..5063
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5079..5096)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5079..5095)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5088..5110)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
       61 tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat
      121 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt
      181 aactactagt atgagtgcga ttaagccaga catgaagatc aaactccgta tggaaggcaa
      241 cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa
      301 acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct
      361 gaccactgca ttccattacg gcaacagggt attcgccaaa tatccagaca acatacaaga
      421 ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga
      481 cgggggcatt tgcattgcca gaaacgacat aacaatggaa ggggacactt tctataataa
      541 agttcgattt tatggtacca actttcccgc caatggtcca gttatgcaga agaagacgct
      601 gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga cgggtgatat
      661 tcatatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta
      721 caaagctaag gagaagggtg tcaagttacc aggctaccac tttgtggacc actgcattga
      781 gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctca
      841 ttctggattg cctgacaatg ccagacgagt gcttgaagag caaaacaaag gaatgatggc
      901 taagattaag tcatttttcg gagtaagatc ttaagcggcc gctaagggtc gacctgcagc
      961 caagcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac ctcagaactc
     1021 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
     1081 agctagggga attcatcgtg actgactgac gatctgcctc gcgcgtttcg gtgatgacgg
     1141 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     1201 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     1261 catgacccag tcacgtagcg atagcggagt gtataattct tgaagacgaa agggcctcgt
     1321 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagg atcctgatgt
     1381 ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg
     1441 atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt
     1501 ggcagcatca cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc
     1561 agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg
     1621 aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
     1681 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
     1741 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
     1801 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
     1861 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     1921 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
     1981 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     2041 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     2101 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     2161 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     2221 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     2281 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg
     2341 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt
     2401 ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgataataag
     2461 cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca gggcagggtc
     2521 gttaaatagc cgcttatgtc tattgctggt ttaccggttt attgactacc ggaagcagtg
     2581 tgaccgtgtg cttctcaaat gcctgaggcc agtttgctca ggctctcccc gtggaggtaa
     2641 taattgacga tatgatcatt tattctgcct cccagagcct gataaaaacg gttaggatcg
     2701 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2761 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2821 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2881 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3061 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3601 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3661 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     3721 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcataa attccgacac
     3781 catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
     3841 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
     3901 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
     3961 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
     4021 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
     4081 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
     4141 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
     4201 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
     4261 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
     4321 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
     4381 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
     4441 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
     4501 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
     4561 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
     4621 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
     4681 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
     4741 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
     4801 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
     4861 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     4921 ggaaagcggg cagtgagcgc aacgcaacta atgtaagtta gctcactcat taggcacccc
     4981 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     5041 ttcacacagg aaacagctat gaccatgatt acggattcac tggccgtcgt tttacaacgt
     5101 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5161 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgc
//
