LOCUS       sequence-135800-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit (AaPurS); hypothetical protein with 
            N-terminal His6 tag (H6AaOrfY), synthetic gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK520
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..26)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     126..145
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             406..423
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(846..862)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(846..862)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(859..881)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    870..886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(894..900,901..918,919..924))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    939..960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
       61 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
      121 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgaaggtac gtgtaaccgt
      181 catgctgaag gacggtgttc tggacccgca gggcaaggcc atcagccacg cattgcagac
      241 acttggtttt gaaggcgtgc acgatgtgcg cgtgggtaag gtgattgaac tggatctgcc
      301 gggtgccaaa gccgaggaag cccagaaaaa ggccgatgcc atggcgcgtg atctgctagc
      361 caacctggtg attgaagatt actctgtaga ggtggtggaa tgagccacca ccaccaccac
      421 cacaagcgca cccttcttat agcgctggca ttggcatgcg cggcaccggg cttggcccat
      481 gcccagcgca ttgcgcccat gaaggcaggt aactttgccc gcctttgtac gcgcgccact
      541 agtgctggca tttgcgatgc ctatattggc ggcctgacgg atggggtctc tctttccaaa
      601 atcaatgatc ggaacgaggg ggatccttct gcgcctgctg gtttttgtgt tcccgctaca
      661 gaaaccatgc ccgttatgcg ctccaaggta cttacctggc tgaaagcgca ttctgaaaca
      721 ctggacaagc cggttggtga aagtgttttt gccgccctgc acgcctctta tccgtgcagt
      781 gtgaaaaaat gaaagcagca attatcgtat tccccggtac cctcgagaag cttggcgtaa
      841 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      901 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      961 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1021 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcct
//
