LOCUS       sequence-135801-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit (AaPurS); hypothetical protein with 
            N-terminal His6 tag (H6AaOrfY), synthetic gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK520
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(399..416)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(677..696)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     796..818
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ggtaccgggg aatacgataa ttgctgcttt cattttttca cactgcacgg ataagaggcg
       61 tgcagggcgg caaaaacact ttcaccaacc ggcttgtcca gtgtttcaga atgcgctttc
      121 agccaggtaa gtaccttgga gcgcataacg ggcatggttt ctgtagcggg aacacaaaaa
      181 ccagcaggcg cagaaggatc cccctcgttc cgatcattga ttttggaaag agagacccca
      241 tccgtcaggc cgccaatata ggcatcgcaa atgccagcac tagtggcgcg cgtacaaagg
      301 cgggcaaagt tacctgcctt catgggcgca atgcgctggg catgggccaa gcccggtgcc
      361 gcgcatgcca atgccagcgc tataagaagg gtgcgcttgt ggtggtggtg gtggtggctc
      421 attccaccac ctctacagag taatcttcaa tcaccaggtt ggctagcaga tcacgcgcca
      481 tggcatcggc ctttttctgg gcttcctcgg ctttggcacc cggcagatcc agttcaatca
      541 ccttacccac gcgcacatcg tgcacgcctt caaaaccaag tgtctgcaat gcgtggctga
      601 tggccttgcc ctgcgggtcc agaacaccgt ccttcagcat gacggttaca cgtaccttca
      661 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
      721 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
      781 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tca
//
