LOCUS       sequence-135807-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 hypothetical protein in purSyQL 
            cluster with C-terminal His6 tag (AaOrfYH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK530
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             417..434
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(487..505)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      501..548
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      585..1013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(672..691)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     882..903
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggc aaagcgcacc cttcttatag
       61 cgctggcatt ggcatgcgcg gcaccgggct tggcccatgc ccagcgcatt gcgcccatga
      121 aggcaggtaa ctttgcccgc ctttgtacgc gcgccactag tgctggcatt tgcgatgcct
      181 atattggcgg cctgacggat ggggtctctc tttccaaaat caatgatcgg aacgaggggg
      241 atccttctgc gcctgctggt ttttgtgttc ccgctacaga aaccatgccc gttatgcgct
      301 ccaaggtact tacctggctg aaagcgcatt ctgaaacact ggacaagccg gttggtgaaa
      361 gtgtttttgc cgccctgcac gcctcttatc cgtgcagtgt gaaaaaatca ctcgagcacc
      421 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      481 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      541 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
      601 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      661 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      721 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      781 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      841 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
      901 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
      961 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     1021 aatatt
//
