LOCUS       sequence-135809-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 hypothetical protein in purSyQL 
            cluster with C-terminal His6 tag (AaOrfYH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK530
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             435..457
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(488..507)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(489..507)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgatttt
       61 ttcacactgc acggataaga ggcgtgcagg gcggcaaaaa cactttcacc aaccggcttg
      121 tccagtgttt cagaatgcgc tttcagccag gtaagtacct tggagcgcat aacgggcatg
      181 gtttctgtag cgggaacaca aaaaccagca ggcgcagaag gatccccctc gttccgatca
      241 ttgattttgg aaagagagac cccatccgtc aggccgccaa tataggcatc gcaaatgcca
      301 gcactagtgg cgcgcgtaca aaggcgggca aagttacctg ccttcatggg cgcaatgcgc
      361 tgggcatggg ccaagcccgg tgccgcgcat gccaatgcca gcgctataag aagggtgcgc
      421 tttgccatgg tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt
      481 gtggtctccc tatagtgagt cgtattaatt tcgcgggatc gagatctcgg gcagcgttgg
      541 gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg
      601 gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg
      661 ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc
      721 gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc
      781 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg
      841 accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca
      901 acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg
      961 tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac
     1021 caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
//
