LOCUS       sequence-135813-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 hypothetical protein in purSyQL 
            cluster (AaOrfY).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK531
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             432..449
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(502..520)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      516..563
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      600..1028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(687..706)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     897..918
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggc aaagcgcacc cttcttatag
       61 cgctggcatt ggcatgcgcg gcaccgggct tggcccatgc ccagcgcatt gcgcccatga
      121 aggcaggtaa ctttgcccgc ctttgtacgc gcgccactag tgctggcatt tgcgatgcct
      181 atattggcgg cctgacggat ggggtctctc tttccaaaat caatgatcgg aacgaggggg
      241 atccttctgc gcctgctggt ttttgtgttc ccgctacaga aaccatgccc gttatgcgct
      301 ccaaggtact tacctggctg aaagcgcatt ctgaaacact ggacaagccg gttggtgaaa
      361 gtgtttttgc cgccctgcac gcctcttatc cgtgcagtgt gaaaaaatga aagcttgcgg
      421 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      481 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      541 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
      601 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      661 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      721 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
      781 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
      841 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
      901 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
      961 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     1021 cgcgaatttt aacaaa
//
