LOCUS       sequence-135814-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 hypothetical protein in purSyQL 
            cluster (AaOrfY).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK531
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             446..468
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(499..518)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(500..518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa
       61 gctttcattt tttcacactg cacggataag aggcgtgcag ggcggcaaaa acactttcac
      121 caaccggctt gtccagtgtt tcagaatgcg ctttcagcca ggtaagtacc ttggagcgca
      181 taacgggcat ggtttctgta gcgggaacac aaaaaccagc aggcgcagaa ggatccccct
      241 cgttccgatc attgattttg gaaagagaga ccccatccgt caggccgcca atataggcat
      301 cgcaaatgcc agcactagtg gcgcgcgtac aaaggcgggc aaagttacct gccttcatgg
      361 gcgcaatgcg ctgggcatgg gccaagcccg gtgccgcgca tgccaatgcc agcgctataa
      421 gaagggtgcg ctttgccatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga
      481 gggaaaccgt tgtggtctcc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg
      541 ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg
      601 ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg
      661 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt
      721 ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg
      781 atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag
      841 cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt
      901 ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc
      961 atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacg
//
