LOCUS       sequence-135815-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of ZapB-mEos2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Buss J, Coltharp C, Shtengel G, Yang X, Hess H, Xiao J
  TITLE     A multi-layered protein network stabilizes the Escherichia coli 
            FtsZ-ring and modulates constriction dynamics.
  JOURNAL   PLoS Genet. 2015 Apr 7;11(4):e1005128. doi: 
            10.1371/journal.pgen.1005128. eCollection 2015 Apr.
  PUBMED    25848771
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2015-04-7"; volume: "11(4):e1005128. doi"; pages: " 
            10.1371/journal.pgen.1005128. eCollection 2015 Apr"
COMMENT     Sequence Label: pJB045 (ZapB-mEos2)
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(70..750)
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 ctggatctat caacaggagt ccaagctcag ctaattaagc ttggctgcag gtcgaccctt
       61 agcggccgct tatcgtctgg cattgtcagg caatccagaa tgagcaacag catgctcata
      121 cagcttaacc ttgttgtaat ctttgtcatg gcttaaaatc tcaatgcagt ggtccacaaa
      181 gtggtagcct ggtaacttga cacccttctc cttagctttg taagtagttc tgaagtcaca
      241 tcggtaatgg gcatttcctt caagcaacaa agccatatga atatcacccg tcagcactcc
      301 atcacgcaca tacattttct cagtggaggg ctcccatttc agcgtcttct tctgcataac
      361 tggaccattg gcgggaaagt tggtaccata aaatcgaact ttattataga aagtgtcccc
      421 ttccattgtt atgtcgtttc tggcaatgca aatgcccccg tcttcgaaag tcaagcttcg
      481 ttcccacgaa taccccttag gaaacgactg cttaaaatag tcttgtatgt tgtctggata
      541 tttggcgaat accctgttgc cgtaatggaa tgcagtggtc aggatatcaa aggcaaaagg
      601 cagaggtccg ccctctttga cttcaagatc catactctgt tttccctcaa aaggcttgcc
      661 tgtaccatct ccgtcgatca caaagtggtg cccgtttacg ttgccttcca tacggagttt
      721 gatcttcatg tctggcttaa tcgcactcat gaattcgcca gaaccagcag cggagccagc
      781 ggatccgacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc agccgttctg
      841 ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct gggcattttg
      901 aacttcctgc gacagtgagt tgtttttt
//
