LOCUS       sequence-135818-       10409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the N-terminal extracellular region of the PlexinB1 
            protein following proteolytic cleavage (ie, short), C-terminally 
            fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 10409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnb1(S)-AP-His
FEATURES             Location/Qualifiers
     source          1..10409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      4038..4047
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6989..7006
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7032..7153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7069..7088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7123..7142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7191..7208)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7362..7950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7442..7461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8121..8912,8913..8981))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8744..8763
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8982..9086)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9154..9172
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9210..9232)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9234..9254)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9239..9569
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9420..9555
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9482..9501
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9706..9725
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9792..10247)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9929..9950)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10141..10160
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10373..10395
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10387..10404
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10388..10404
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgtctgtcct
     1621 cggcccagtt cttctccagg tgttctgggc cgggtgtgtc gtcaccctgc ggtcccctct
     1681 gccagctgct ttcactgcca atggcacaca tctacaacac ttggcaaggg accccaccac
     1741 aggtaccctc tatgtagggg ccaccaactt cctgttccag ttgagccctg ggctgcagct
     1801 ggaagccgtg gtgtccacgg gccctgtgaa tgacagccgg gattgcctgc cacctgtgat
     1861 acctgatgaa tgtccccaag cccagcctac taacaaccct aaccagctgc tcctggtgag
     1921 cccagaggct ctggtggtgt gtgggagcat acaccagggc atctgtgagc tacggagcct
     1981 gggacagatc aggcagctgc tgctacggcc agagcgacct ggggacaccc agtatgtggc
     2041 tgcaaatgac cctgcagtca gtacagtggg gctggtggcc cagggattgg taggggagcc
     2101 cctcctgttt gtggggcggg gatacaccag caggggtgta ggtggtggga ttcctcccat
     2161 tacaacccga gccctgcgac caccggaccc ccaagctgcc ttctcttatg aagaaacagc
     2221 caagttagca gtgggccgcc tgtccgagta cagccaccac ttcgtgagtg cctttgtacg
     2281 cggggccagt gcatacttcc tgttcttgcg gcgagacctg aaggcccctt ctagagcttt
     2341 ccgtgcctat gtgtctcgag tgtgccttca ggaccagcac tactactctt atgtggaatt
     2401 gcccctggcc tgccagggtg gtcgttacgg tcttatccag gctgcagctg tagccacgtc
     2461 caaggaggtg gcccgtgggg acgtactctt tgcagctttc tcctcagtgg ctcctcccac
     2521 tgtggattgg cccctgtcag catctactgg ggcatctgga acctctgtgc tctgtgcctt
     2581 ccccctggat gaggtagacc agcttgctaa ttacactcga gatgcctgtt atactcggga
     2641 aggccgtgct gagaacggga ccaaggttgc tgacattgca tacgatgtcc tttccgactg
     2701 tgcgcagcta ccagtggaca ccccggatgc ttttccatgt ggctctgacc acacacccag
     2761 tcccatggtc agctgtgtcc ctttggaagc cacgccaatt ctggagctac caggggttca
     2821 gctaacagct gtggctgtca ccatggagga tggacacact attgctttcc tgggtgacag
     2881 tcaaggacag ttgcataggg tctacttagg ccctggaaga agtgctgccc catattctaa
     2941 acagagcatc cagccggggt ctcctgtgaa cagagatctt acctttgatg gtacctttga
     3001 gcatctctat gtagcaactc agactactct tgtgaaggtt cctgtggctc cttgtgctca
     3061 gcatctggac tgtgactctt gccttgctca cagggaccct tattgcggat ggtgtgtgct
     3121 cctgggcagg tgtagtcgcc ggtcggagtg ctcaagggac cagggcccag agcagtggct
     3181 gtggagcttc cagccggaac tgggctgtct tcgagtggtg gccgtgagcc ctgccaatat
     3241 cagtcgggaa gagaggaggg aggttttctt gtcagtgcca ggcctgccat ctctctggcc
     3301 aggggagtca tatttctgct actttggaga ccaacagagt cctgctctac tgaccagttc
     3361 tggtgtgatg tgtccctccc cagaccccag tgaggctcca gtgctgcaga gaggagccga
     3421 ccatatctct gtgaacgtgg agctcaggtt tggtgccgtg gtgatcgcca gcacctccct
     3481 ctccttctat gactgcgtgg cagttactgc gtcttcccca tctgcaccgt gccgggcctg
     3541 tgtgagcagc cgctggggct gtaactggtg tgtgtggcag cagctgtgca cacacaaggc
     3601 ctcgtgtgac gctgggccta tggtggcaag ccaacagagc ccactccttc ccctaatccc
     3661 tcctgcaagg gatgaactca cccctttccc acccacagtc ccccaaacca cggtcactcc
     3721 tacccccaac agcttcccaa tagagcctag ggctccctcc acagcctcag atgtcctacc
     3781 tggggccaag ccttcccggc tcagcctctg gggcccatgg gcaggtcctg gccccatact
     3841 ttcccctact tccacagagt cacctcttca tgagaagccc cttcctcctg acccccctac
     3901 catacctgga accactgtcc ctgcccccac tggcttggga ccatcgacca cacctgagga
     3961 cctcttggcc tcctacccat tcccctcaga tgcagctgca gtgtcccctg cagagcctgg
     4021 ccctgaggct ctgccttcca tggtggctct ggaccagccc cctggcactg ttccagacac
     4081 tactttccca ggggcccctg gctccatgaa gcccgttctg gattggctca ccaaaggagg
     4141 cggcgagctg cccgaggcgg atgagtggat ggggggtgac acgcccgcct tctccacttc
     4201 cacactcctc tcaggtgatg gagactcagc agagcacgag ggccctcctg cccccctcat
     4261 cctcctgtcc agcctcgact accagtacga cacccccggg ctctgggagc tgggagaggt
     4321 gaatcagagg gtgagctcct gcccctgtgt ggagaccgtc cagggctcct tgctgatacc
     4381 ggtccatgtg gaacgcgaag tccagcttcg aggcaggaac ctgtggcttt tccaggatgg
     4441 cccgaggagc agcgagtgtg tgctggagct agggagtcgg gaggtggctg tggaggctca
     4501 ggtggagtgt gcgccgcctc cagatgtctg gtgccacatc aagtgccagc agcatcagtt
     4561 cagctatgaa gctttgaagc cagaactgca ggtggggctg ttcctgcgtt gggcaggcgg
     4621 tctgcgcgtg gacagtgccg atgggctgca tgtggtgttg tatgactgct ctgtgggaca
     4681 tggggactgc agccgctgcc aaactgccat gcctcagtac gactgtgtgt ggtgtgaggg
     4741 ggagcgtccg cgttgtgtgg cccgggaagc ctgtaatgaa gccgagactg tggccactca
     4801 gtgccccgca cctctcattc actcggtgga tccactgact ggacctatag atggaggcac
     4861 ccgtgtcact atcaggggct ccaacctggg ccaacatgtg caggatgtcc tggacatggt
     4921 cagagtggcc ggagttccct gcgctgtgga tgctggggag tatgatgtct ctagtagtct
     4981 tgtgtgcatc actggagcca gcggggagga ggtgactggc actgtggcag tggaggtgcc
     5041 tggaagagga cacggtgtct cagagttcag ctttgcctat caggatccaa aagtacactc
     5101 catcttccca gcccgtggcc ctagagctgg aggtacccgc cttaccctgc atggttctaa
     5161 gctcctgact ggacggctag aggacatccg tgtggtggtt ggagaccagc cttgccacct
     5221 gctcctggag cagcagtctg agcagctaca ctgtgagacc ggcccatacc ctgtgcctgc
     5281 tgaacttcca gtgactgtct tgtttggggc cactgagcgg aggcttcagc acggccaatt
     5341 caagtataca tcagacccca atgtcacctc agtgggcccc tccaagagct tcttcagcgg
     5401 aggacgtgag atatgggtcc gcggccagga tcttgatgtg gtacagaggc caagaatccg
     5461 agtgaccgtg gtcccaagac agcatggcca ggggcttgca ggcgcgcctg gatcttccgg
     5521 aatcatccca gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct
     5581 gggtgccgcc aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct
     5641 gggcgatggg atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa
     5701 ggacaaactg gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc
     5761 caagacatac aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct
     5821 gtgcggggtc aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca
     5881 gtgcaacacg acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg
     5941 gaagtcagtg ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta
     6001 cgcccacacg gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca
     6061 ggaggggtgc caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct
     6121 aggtgggggc cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga
     6181 ctacagccaa ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa
     6241 gcgccagggt gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc
     6301 gtctgtgacc catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg
     6361 agactccaca ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag
     6421 caggaacccc cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca
     6481 tgaaagcagg gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag
     6541 ggcgggccag ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca
     6601 cgtcttctcc ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg
     6661 caaggcccgg gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt
     6721 gctcaaggac ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg
     6781 gcagcagtca gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt
     6841 cgcgcgcggc ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca
     6901 cgtcatggcc ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc
     6961 cggcaccacc gacgccgcgc acccgggtca tcatcaccat caccattaat ctagagatct
     7021 tattaaagca gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7081 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7141 tcaatgtatc ttatcatgtc tggtcgactc tagctagact cttccgcttc ctcgctcact
     7201 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7261 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7321 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7381 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7441 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7501 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7561 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7621 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7681 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7741 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7801 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7861 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7921 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7981 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8041 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8101 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8161 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8221 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8281 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8341 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8401 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8461 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8521 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8581 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8641 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8701 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8761 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8821 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8881 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8941 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9001 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9061 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     9121 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     9181 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggaggaatgt
     9241 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca
     9301 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9361 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9421 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9481 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     9541 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     9601 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     9661 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     9721 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     9781 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     9841 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     9901 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     9961 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
    10021 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
    10081 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
    10141 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
    10201 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
    10261 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    10321 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    10381 cgacgttgta aaacgacggc cagtgaatt
//
