LOCUS       sequence-135821-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit (AaPurS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK533
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             297..314
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(367..385)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      381..428
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      465..920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(552..571)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     762..783
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        946..1050
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atggcgaagg tacgtgtaac cgtcatgctg
       61 aaggacggtg ttctggaccc gcagggcaag gccatcagcc acgcattgca gacacttggt
      121 tttgaaggcg tgcacgatgt gcgcgtgggt aaggtgattg aactggatct gccgggtgcc
      181 aaagccgagg aagcccagaa aaaggccgat gctatggcgc gtgatctgct agccaacctg
      241 gtgattgaag attactctgt agaggtggtg gaatgaagct tgcggccgca ctcgagcacc
      301 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      361 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      421 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
      481 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      541 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      601 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      661 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      721 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
      781 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
      841 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
      901 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      961 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1021 atgcttcaat aatattgaaa aaggaagagt atga
//
