LOCUS       sequence-135829-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Kim D, Fiske BP, Birsoy K, Freinkman E, Kami K, Possemato RL, 
            Chudnovsky Y, Pacold ME, Chen WW, Cantor JR, Shelton LM, Gui DY, 
            Kwon M, Ramkissoon SH, Ligon KL, Kang SW, Snuderl M, Vander Heiden 
            MG, Sabatini DM
  TITLE     SHMT2 drives glioma cell survival in ischaemia but imposes a 
            dependence on glycine clearance.
  JOURNAL   Nature. 2015 Apr 16;520(7547):363-7. doi: 10.1038/nature14363. Epub 
            2015 Apr 8.
  PUBMED    25855294
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14363"; journalName: "Nature"; date: "2015-04-16- 
            16"; volume: "520"; issue: "7547"; pages: "363-7"
COMMENT     Sequence Label: SHMT2-RES
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             146..541
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
       61 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
      121 acacgatgat aatatggcca caaccatggt tcctttgtct caagaagaat ccaccctcat
      181 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
      241 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
      301 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
      361 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
      421 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
      481 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
      541 agtcgacgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
      601 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
      661 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
      721 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
      781 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
      841 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagttt
//
