LOCUS       sequence-135831-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 hypothetical protein in purSyQL 
            cluster (AaOrfY).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK546
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        592..610
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    611..635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             744..788
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(826..844)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      840..887
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1011..1030)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta
       61 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat ataccatggc aaagcgcacc cttcttatag cgctggcatt
      181 ggcatgcgcg gcaccgggct tggcccatgc ccagcgcatt gcgcccatga aggcaggtaa
      241 ctttgcccgc ctttgtacgc gcgccactag tgctggcatt tgcgatgcct atattggcgg
      301 cctgacggat ggggtctctc tttccaaaat caatgatcgg aacgaggggg atccttctgc
      361 gcctgctggt ttttgtgttc ccgctacaga aaccatgccc gttatgcgct ccaaggtact
      421 tacctggctg aaagcgcatt ctgaaacact ggacaagccg gttggtgaaa gtgtttttgc
      481 cgccctgcac gcctcttatc cgtgcagtgt gaaaaaatga aagcttgcgg ccgcataatg
      541 cttaagtcga acagaaagta atcgtattgt acacggccgc ataatcgaaa ttaatacgac
      601 tcactatagg ggaattgtga gcggataaca attccccatc ttagtatatt agttaagtat
      661 aagaaggaga tatacatatg gcagatctca attggatatc ggccggccac gcgatcgctg
      721 acgtcggtac cctcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca
      781 tggactcgtc tactagcgca gcttaattaa cctaggctgc tgccaccgct gagcaataac
      841 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      901 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      961 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     1021 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgg
//
