LOCUS       sequence-135832-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 hypothetical protein in purSyQL 
            cluster (AaOrfY).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK546
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(90..137)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     133..151
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(189..233)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    342..366
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(367..385)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             838..860
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    875..899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(900..918)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1006..1025)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
       61 gccaatccgg atatagttcc tcctttcagc aaaaaacccc tcaagacccg tttagaggcc
      121 ccaaggggtt atgctagtta ttgctcagcg gtggcagcag cctaggttaa ttaagctgcg
      181 ctagtagacg agtccatgtg ctggcgttca aatttcgcag cagcggtttc tttaccagac
      241 tcgagggtac cgacgtcagc gatcgcgtgg ccggccgata tccaattgag atctgccata
      301 tgtatatctc cttcttatac ttaactaata tactaagatg gggaattgtt atccgctcac
      361 aattccccta tagtgagtcg tattaatttc gattatgcgg ccgtgtacaa tacgattact
      421 ttctgttcga cttaagcatt atgcggccgc aagctttcat tttttcacac tgcacggata
      481 agaggcgtgc agggcggcaa aaacactttc accaaccggc ttgtccagtg tttcagaatg
      541 cgctttcagc caggtaagta ccttggagcg cataacgggc atggtttctg tagcgggaac
      601 acaaaaacca gcaggcgcag aaggatcccc ctcgttccga tcattgattt tggaaagaga
      661 gaccccatcc gtcaggccgc caatataggc atcgcaaatg ccagcactag tggcgcgcgt
      721 acaaaggcgg gcaaagttac ctgccttcat gggcgcaatg cgctgggcat gggccaagcc
      781 cggtgccgcg catgccaatg ccagcgctat aagaagggtg cgctttgcca tggtatatct
      841 ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc
      901 ctatagtgag tcgtattaat ttcgcgggat cgagatcgat ctcgatcctc tacgccggac
      961 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     1021 tcaccgatgg ggaaga
//
