LOCUS       sequence-135835-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Tang ED, Nunez G, Barr FG, Guan KL
  TITLE     Negative regulation of the forkhead transcription factor FKHR by 
            Akt.
  JOURNAL   J Biol Chem. 1999 Jun 11. 274(24):16741-6.
  PUBMED    10358014
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Jun 11. 274(24):16741-6."
COMMENT     Sequence Label: pcDNA3 HA human NEMO
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             75..101
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             75..101
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             81..107
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 agcttaccat ggcctacccc tacgacgtgc ccgactacgc ctccctcgga tccatgaata
      121 ggcacctctg gaagagccaa ctgtgtgaga tggtgcagcc cagtggtggc ccggcagcag
      181 atcaggacgt actgggcgaa gagtctcctc tggggaagcc agccatgctg cacctgcctt
      241 cagaacaggg cgctcctgag accctccagc gctgcctgga ggagaatcaa gagctccgag
      301 atgccatccg gcagagcaac cagattctgc gggagcgctg cgaggagctt ctgcatttcc
      361 aagccagcca gagggaggag aaggagttcc tcatgtgcaa gttccaggag gccaggaaac
      421 tggtggagag actcggcctg gagaagctcg atctgaagag gcagaaggag caggctctgc
      481 gggaggtgga gcacctgaag agatgccagc agcagatggc tgaggacaag gcctctgtga
      541 aagcccaggt gacgtccttg ctcggggagc tgcaggagag ccagagtcgc ttggaggctg
      601 ccactaagga atgccaggct ctggagggtc gggcccgggc ggccagcgag caggcgcggc
      661 agctggagag tgagcgcgag gcgctgcagc agcagcacag cgtgcaggtg gaccagctgc
      721 gcatgcaggg ccagagcgtg gaggccgcgc tccgcatgga gcgccaggcc gcctcggagg
      781 agaagaggaa gctggcc
//
