LOCUS       sequence-135844-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase glutaminase subunit (AaPurQ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK555
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             814..831
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(884..902)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      898..945
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatga aagcagcaat tatcgtattc
       61 cccggcacaa accgggagcg cgatatggcc attgccctca agcgcgttac agggcaggcc
      121 ccgcgcatga tctggcacca tgaaacagat ctgggagata ccgatctagt cgtgctgcca
      181 ggtggtttca gctatggcga ttatctgcgc tgtggtgcca tggcagcgca ttctcccatc
      241 atggcggcta tcaaagcctt tgcccaaaag ggtggccata ttttgggtgt gtgcaacggc
      301 ttccagattt taacggaaac agggcttctg cccggtgctc tgttgcgcaa cgccaacctg
      361 cgctttctct ctcaggattg ctacctgaag gtggaagcag agaacacacc tttcactcac
      421 aagtggaaga agggtgatgt tttccgcacc cctctggccc atggtgatgg caattacacg
      481 gcagatatcg ccacgctgga acggcttgaa ggtgaaggtc ttgtggcttt ccactatgcc
      541 gatgccaaag gcaatgtgca ggctgatgat gccatcagca accccaatgg cagcatcaac
      601 agcattgcgg gtattctgag cgaaaacaaa cgcatttgcg gcatgatgcc tcatccagaa
      661 gatctggttg atcctctcat gggtggggaa gatggtctgc cattgttcca aggtctggtg
      721 gaggctgtag tccggtgaat aagcctgtaa ccgttgatga atcccttgcc cgtgaattcg
      781 agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      841 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      901 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      961 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1021 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct c
//
