LOCUS       sequence-135845-        1093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase glutaminase subunit (AaPurQ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK555
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             824..846
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(877..896)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(878..896)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattca cgggcaaggg attcatcaac ggttacaggc ttattcaccg
      121 gactacagcc tccaccagac cttggaacaa tggcagacca tcttccccac ccatgagagg
      181 atcaaccaga tcttctggat gaggcatcat gccgcaaatg cgtttgtttt cgctcagaat
      241 acccgcaatg ctgttgatgc tgccattggg gttgctgatg gcatcatcag cctgcacatt
      301 gcctttggca tcggcatagt ggaaagccac aagaccttca ccttcaagcc gttccagcgt
      361 ggcgatatct gccgtgtaat tgccatcacc atgggccaga ggggtgcgga aaacatcacc
      421 cttcttccac ttgtgagtga aaggtgtgtt ctctgcttcc accttcaggt agcaatcctg
      481 agagagaaag cgcaggttgg cgttgcgcaa cagagcaccg ggcagaagcc ctgtttccgt
      541 taaaatctgg aagccgttgc acacacccaa aatatggcca cccttttggg caaaggcttt
      601 gatagccgcc atgatgggag aatgcgctgc catggcacca cagcgcagat aatcgccata
      661 gctgaaacca cctggcagca cgactagatc ggtatctccc agatctgttt catggtgcca
      721 gatcatgcgc ggggcctgcc ctgtaacgcg cttgagggca atggccatat cgcgctcccg
      781 gtttgtgccg gggaatacga taattgctgc tttcatatgt atatctcctt cttaaagtta
      841 aacaaaatta tttctagagg gaaaccgttg tggtctccct atagtgagtc gtattaattt
      901 cgcgggatcg agatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc
      961 ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca
     1021 ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca
     1081 acatgaatgg tct
//
