LOCUS       sequence-135849-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Sema3A protein (truncated at cleavage site P3; ie, 
            long and missing exon 3), C-terminally fused to alkaline phosphatase
            + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3a(L, del3)-AP-His
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ataagatctc tagattaatg gtgatggtga tgatgacccg ggtgcgcggc gtcggtggtg
       61 ccggcggggg gcgccaggtc gcaggcggtg tagggctcca ggcaggcggc gaaggccatg
      121 acgtgcgcta tgaaggtctg ctcctgcacg ccgtgaacca ggtgcgcctg cgggccgcgc
      181 gcgaacaccg ccacgtcctc gcctgcgtgg gtctcttcgt ccaggggcac tgctgactgc
      241 tgccgatact cggggctccc gctctcgctc tcggtaacat ccggccgggc gccgtccttg
      301 agcacatagc ctggaccgtt tccgtatagg aggaccgtgt aggccttcct gtcccgggcc
      361 ttgccagggg ccagcccgaa gatggagctc cctcgcaggg ggtagcctcc gaaggagaag
      421 acgtgggagt ggtcggcagt gacgaggctc agcgtgtcct cctcgctggt gagctggccc
      481 gccctctcaa tggcgtcgtc gaacatgatc gtctcagtca gtgcccggta agccctgctt
      541 tcatgatgac catggtcgat gcgaccaccc tccacgaaga ggaagaagcc gcgggggttc
      601 ctgctcagca ggcgcagggc agcctctgtc atctccatca gggaggggtc cagtgtggag
      661 tctcggtgga tctcgtattt catgtctcca ggctcaaaga gacccatgag atgggtcaca
      721 gacgggtcca gggaagcctg catgagctca gtgcggttcc acacataccg ggcaccctgg
      781 cgcttcgcca gccattcctg caccagat
//
